Modulation of chemokine production and expression of adhesion molecules in renal tubular epithelial and endothelial cells by catecholamines

• Verfasst von: Kapper, Silke [VerfasserIn]
• Verfasst von: Beck, Grietje [VerfasserIn]
• Verfasst von: Riedel, Saskia [VerfasserIn]
• Verfasst von: Prem, Katharina [VerfasserIn]
• Verfasst von: Haak, Markus [VerfasserIn]
• Verfasst von: Woude, Fokko J. van der [VerfasserIn]
• Verfasst von: Yard, Benito A. [VerfasserIn]
• Titel: Modulation of chemokine production and expression of adhesion molecules in renal tubular epithelial and endothelial cells by catecholamines
• Verf.angabe: Silke Kapper, Grietje Beck, Saskia Riedel, Katharina Prem, Markus Haak, Fokko J. van der Woude and Benito A. Yard
• Jahr: 2002
• Umfang: 8 S.
• Fussnoten: Gesehen am 29.06.2022
• Titel Quelle: Enthalten in: Transplantation
• Ort Quelle: Hagerstown, Md. : Lippincott Williams & Wilkins, 1963
• Jahr Quelle: 2002
• Band/Heft Quelle: 74(2002), 2, Seite 253-260
• ISSN Quelle: 1534-6080
• DOI: doi:10.1097/00007890-200207270-00017
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Catecholamines
• Sach-SW: Cell Adhesion Molecules
• Sach-SW: Cells, Cultured
• Sach-SW: Chemokines
• Sach-SW: Dopamine
• Sach-SW: E-Selectin
• Sach-SW: Endothelium, Vascular
• Sach-SW: Epithelial Cells
• Sach-SW: Humans
• Sach-SW: Intercellular Adhesion Molecule-1
• Sach-SW: Kidney Tubules
• Sach-SW: Tumor Necrosis Factor-alpha
• Sach-SW: Vascular Cell Adhesion Molecule-1
• K10plus-PPN: 1808522095

Abstract

BACKGROUND: The present study was conducted to investigate whether catecholamines influence the production of chemokines and adhesion molecules in proximal tubular epithelial cells (PTECs) and endothelial cells. METHODS: PTECs and human umbilical vein endothelial cells (HUVECs) were stimulated with various concentrations of dopamine (DA), adrenaline (AD), or noradrenaline (NA), and the production of interleukin (IL)-8, ENA-78, and Gro-alpha was assessed by ELISA. The influence of catecholamine pretreatment on tumor necrosis factor (TNF)-alpha-mediated production of these chemokines and the expression of adhesion molecules was also tested. RESULTS: In PTECs, DA inhibited the production of all three chemokines in a dose-dependent fashion. Although inhibition in ENA-78 and Gro-alpha production was also found in HUVECs, IL-8 production was up-regulated in these cells. Increased IL-8 secretion was predominantly observed at the apical site of the cells. In AD or NA stimulated cells, the production of Gro-alpha was increased in PTECs and decreased in HUVECs. Down-regulation in IL-8 production was also observed after AD but not after NA stimulation of both cell types. Interestingly, TNF-alpha-mediated up-regulation in intercellular adhesion molecule 1, vascular cell adhesion molecule (VCAM), and E-selectin was delayed in DA-pretreated HUVECs but not in PTECs. The influence of DA, but not AD or NA, on chemokine production was completely prevented by the addition of N-acetylcysteine. CONCLUSIONS: This study demonstrates that catecholamines differentially influence chemokine production and indicates that DA may have anti-inflammatory properties because it delays the expression of adhesion molecules and inhibits the production of chemokines in PTECs and endothelial cells under basal and inflammatory conditions.