Status: Bibliographieeintrag
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| Verfasst von: | Hoffmann, Sabrina [VerfasserIn]  |
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| | Wabnitz, Guido H. [VerfasserIn] |
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| | Samstag, Yvonne [VerfasserIn] |
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| | Moldenhauer, Gerhard [VerfasserIn] |
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| | Ludwig, Thomas [VerfasserIn] |
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| Titel: | Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lymphocyte and cancer cell interaction by single-cell force spectroscopy |
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| Verf.angabe: | Sabrina C. Hoffmann, Guido H. Wabnitz, Yvonne Samstag, Gerhard Moldenhauer and Thomas Ludwig |
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| E-Jahr: | 2011 |
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| Jahr: | [1 May 2011] |
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| Umfang: | 9 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | First published: 08 March 2011 ; Gesehen am 11.07.2022 |
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| Titel Quelle: | Enthalten in: International journal of cancer |
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| Ort Quelle: | Bognor Regis : Wiley-Liss, 1966 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 128(2011), 9 vom: Mai, Seite 2096-2104 |
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| ISSN Quelle: | 1097-0215 |
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| Abstract: | The atomic force microscopy (AFM) is a powerful tool to analyze forces generated on cellular interactions on the single-cell level. This highly sensitive device can record changes in force in the pico-Newton range, which equals single molecule bonds. Here, we have used single-cell force spectroscopy by AFM to investigate the interaction between T cells and tumor cells that is induced by the bispecific antibody HEA125xOKT3 (specificity anti-EpCAMxCD3). We show that HEA125xOKT3 induces a specific increase in adhesion force between T cells and cancer cells. The adhesive force that is generated on cell-cell contact is dependent on the applied force on initial contact and the duration of this initial contact. In summary, HEA125xOKT3 has been found to mediate contact formation by distinct processes. It induces direct cell-cell interaction, which results in the activation of T-cell signaling, facilitates the formation of supramolecular activation clusters and ultimately of an immune synapse. |
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| DOI: | doi:10.1002/ijc.25556 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/ijc.25556 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.25556 |
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| | DOI: https://doi.org/10.1002/ijc.25556 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | atomic force microscopy |
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| | bispecific antibody |
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| | single-cell force spectroscopy |
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| | synapse formation |
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| | T-cell immunology |
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| K10plus-PPN: | 1809692032 |
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| Verknüpfungen: | → Zeitschrift |
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Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lymphocyte and cancer cell interaction by single-cell force spectroscopy / Hoffmann, Sabrina [VerfasserIn]; [1 May 2011] (Online-Ressource)
68939944
