Serotonin receptor diversity in the human colon

• Verfasst von: Kapeller, Johannes [VerfasserIn]
• Verfasst von: Möller, Dorothee [VerfasserIn]
• Verfasst von: Lasitschka, Felix [VerfasserIn]
• Verfasst von: Autschbach, Frank [VerfasserIn]
• Verfasst von: Hovius, Ruud [VerfasserIn]
• Verfasst von: Rappold, Gudrun [VerfasserIn]
• Verfasst von: Brüss, Michael [VerfasserIn]
• Verfasst von: Gershon, Michael D. [VerfasserIn]
• Verfasst von: Niesler, Beate [VerfasserIn]
• Titel: Serotonin receptor diversity in the human colon
• Titelzusatz: expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E
• Verf.angabe: Johannes Kapeller, Dorothee Möller, Felix Lasitschka, Frank Autschbach, Ruud Hovius, Gudrun Rappold, Michael Brüss, Michael D. Gershon, and Beate Niesler
• Jahr: 2011
• Umfang: 13 S.
• Fussnoten: Published online October 19, 2010 ; Gesehen am 11.07.2022
• Titel Quelle: Enthalten in: The journal of comparative neurology
• Ort Quelle: New York, NY [u.a.] : Wiley-Liss, 1891
• Jahr Quelle: 2011
• Band/Heft Quelle: 519(2011), 3, Seite 420-432
• ISSN Quelle: 1096-9861
• DOI: doi:10.1002/cne.22525
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Serotonin receptor diversity in the human colon. - 2011
• Sach-SW: 5-HT receptor
• Sach-SW: 5-HT3
• Sach-SW: antibody
• Sach-SW: gastrointestinal tract
• Sach-SW: ligand-gated ion channel
• K10plus-PPN: 1809696356

Abstract

Since the first description of 5-HT3 receptors more than 50 years ago, there has been speculation about the molecular basis of their receptor heterogeneity. We have cloned the genes encoding novel 5-HT3 subunits 5-HT3C, 5-HT3D, and 5-HT3E and have shown that these subunits are able to form functional heteromeric receptors when coexpressed with the 5-HT3A subunit. However, whether these subunits are actually expressed in human tissue remained to be confirmed. In the current study, we performed immunocytochemistry to locate the 5-HT3A as well as the 5-HT3C, 5-HT3D, and 5-HT3E subunits within the human colon. Western blot analysis was used to confirm subunit expression, and RT-PCR was employed to detect transcripts encoding 5-HT3 receptor subunits in microdissected tissue samples. This investigation revealed, for the first time, that 5-HT3C, 5-HT3D, and 5-HT3E subunits are coexpressed with 5-HT3A in cell bodies of myenteric neurons. Furthermore, 5-HT3A and 5-HT3D were found to be expressed in submucosal plexus of the human large intestine. These data provide a strong basis for future studies of the roles that specific 5-HT3 receptor subtypes play in the function of the enteric and central nervous systems and the contribution that specific 5-HT3 receptors make to the pathophysiology of gastrointestinal disorders such as irritable bowel syndrome and dyspepsia. J. Comp. Neurol. 519:420-432, 2011. © 2010 Wiley-Liss, Inc.