| |  Online-Ressource |
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| Verfasst von: | Kupke, Thomas [VerfasserIn]  |
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| | Di Cecco, Leontina [VerfasserIn] |
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| | Müller, Hans-Michael [VerfasserIn] |
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| | Neuner, Annett [VerfasserIn] |
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| | Adolf, Frank [VerfasserIn] |
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| | Wieland, Felix T. [VerfasserIn] |
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| | Nickel, Walter [VerfasserIn] |
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| | Schiebel, Elmar [VerfasserIn] |
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| Titel: | Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication |
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| Verf.angabe: | Thomas Kupke, Leontina Di Cecco, Hans-Michael Müller, Annett Neuner, Frank Adolf, Felix Wieland, Walter Nickel and Elmar Schiebel |
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| E-Jahr: | 2011 |
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| Jahr: | 22 July 2011 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 11.07.2022 |
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| Titel Quelle: | Enthalten in: European Molecular Biology OrganizationThe EMBO journal |
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| Ort Quelle: | Heidelberg : EMBO Press, 1982 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 30(2011), 16, Seite 3337-3352 |
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| ISSN Quelle: | 1460-2075 |
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| Abstract: | Spindle pole bodies (SPBs), like nuclear pore complexes, are embedded in the nuclear envelope (NE) at sites of fusion of the inner and outer nuclear membranes. A network of interacting proteins is required to insert a cytoplasmic SPB precursor into the NE. A central player of this network is Nbp1 that interacts with the conserved integral membrane protein Ndc1. Here, we establish that Nbp1 is a monotopic membrane protein that is essential for SPB insertion at the inner face of the NE. In vitro and in vivo studies identified an N-terminal amphipathic α-helix of Nbp1 as a membrane-binding element, with crucial functions in SPB duplication. The karyopherin Kap123 binds to a nuclear localization sequence next to this amphipathic α-helix and prevents unspecific tethering of Nbp1 to membranes. After transport into the nucleus, Nbp1 binds to the inner nuclear membrane. These data define the targeting pathway of a SPB component and suggest that the amphipathic α-helix of Nbp1 is important for SPB insertion into the NE from within the nucleus. |
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| DOI: | doi:10.1038/emboj.2011.242 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/emboj.2011.242 |
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| | Volltext: https://www.embopress.org/doi/full/10.1038/emboj.2011.242 |
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| | DOI: https://doi.org/10.1038/emboj.2011.242 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | amphipathic helix |
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| | in-plane membrane anchor |
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| | Kap123 |
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| | Nbp1 |
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| | spindle pole body duplication |
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| K10plus-PPN: | 1809706092 |
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| Verknüpfungen: | → Zeitschrift |
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Targeting of Nbp1 to the inner nuclear membrane is essential for spindle pole body duplication / Kupke, Thomas [VerfasserIn]; 22 July 2011 (Online-Ressource)
