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Status: Bibliographieeintrag

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Verfasst von:Miller-Lazić, Daliborka [VerfasserIn]   i
 Alborzi, Farnoosh [VerfasserIn]   i
 Marcuzzi, Gian Paolo [VerfasserIn]   i
 Angel, Peter [VerfasserIn]   i
 Heß, Jochen [VerfasserIn]   i
 Pfister, Herbert [VerfasserIn]   i
 Akgül, Baki [VerfasserIn]   i
Titel:Enhanced StefinA and Sprr2 expression during papilloma formation in HPV8 transgenic mice
Verf.angabe:Daliborka Lazić, Farnoosh Alborzi, Gian Paolo Marcuzzi, Peter Angel, Jochen Hess, Herbert Pfister, Baki Akgül
E-Jahr:2011
Jahr:1 March 2011
Umfang:7 S.
Fussnoten:Gesehen am 13.07.2022
Titel Quelle:Enthalten in: Journal of dermatological science
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1990
Jahr Quelle:2011
Band/Heft Quelle:62(2011), 2, Seite 84-90
ISSN Quelle:1873-569X
Abstract:Background - The human papillomavirus type 8 (HPV8) is associated with the development of non-melanoma skin cancer. Transgenic mice expressing the complete early gene region of HPV8 (E6/E7/E1/E2/E4=CER) or E6 separately under the control of the keratin14 promoter spontaneously developed papillomas characterized by varying degrees of epidermal dysplasia. Papilloma growth could be synchronized by a single UVA/B irradiation of the skin, which led to the development of papillomas within three weeks. - Objective - The objective of this study was to identify alterations in cellular gene expression correlated with HPV8 oncogene expression in transgenic mice. - Methods - We applied global gene expression profiling by microarray analysis and confirmed deregulation of cellular genes by qRT-PCR and immunohistochemical analysis. - Results - By comparison of non-lesional HPV8-CER skin with skin of the parental mouse strain FVB/n, two cellular genes, namely StefinA and Sprr2, coding for precursor proteins of the cornified envelope, were predicted to be strongly upregulated in transgenic skin, which could be confirmed in subsequent qRT-PCR experiments. StefinA and Sprr2 mRNA expression was enhanced until day 7 after UV treatment with higher levels in HPV8 positive skin. While the expression of both genes returned to a normal level in the course of epidermis regeneration in wt mice, the expression persisted elevated in hyperplastic transgenic skin. Staining of an UV induced papilloma of FVB/n wt mouse revealed also strong expression of StefinA and Sprr2 indicating that upregulation in later stages of papilloma formation is independent of HPV8. - Conclusion - In non-lesional HPV8-CER transgenic skin StefinA and Sprr2 were found to be indirect/direct transcriptional targets of HPV8.
DOI:doi:10.1016/j.jdermsci.2011.02.006
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.jdermsci.2011.02.006
 Volltext: https://www.sciencedirect.com/science/article/pii/S0923181111000594
 DOI: https://doi.org/10.1016/j.jdermsci.2011.02.006
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:HPV8
 Skin papilloma development
 Transgenic mice
K10plus-PPN:1810087546
Verknüpfungen:→ Zeitschrift

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