Intrinsically disordered intracellular domains control key features of the mechanically-gated ion channel PIEZO2

• Verfasst von: Verkest, Clément [VerfasserIn]
• Verfasst von: Schaefer, Irina [VerfasserIn]
• Verfasst von: Nees, Timo A. [VerfasserIn]
• Verfasst von: Wang, Na [VerfasserIn]
• Verfasst von: Jegelka, Juri M. [VerfasserIn]
• Verfasst von: Taberner, Francisco J. [VerfasserIn]
• Verfasst von: Lechner, Stefan [VerfasserIn]
• Titel: Intrinsically disordered intracellular domains control key features of the mechanically-gated ion channel PIEZO2
• Verf.angabe: Clement Verkest, Irina Schaefer, Timo A. Nees, Na Wang, Juri M. Jegelka, Francisco J. Taberner & Stefan G. Lechner
• E-Jahr: 2022
• Jahr: 15 March 2022
• Umfang: 14 S.
• Fussnoten: Gesehen am 18.07.2022
• Titel Quelle: Enthalten in: Nature Communications
• Ort Quelle: [London] : Nature Publishing Group UK, 2010
• Jahr Quelle: 2022
• Band/Heft Quelle: 13(2022), Artikel-ID 1365, Seite 1-14
• ISSN Quelle: 2041-1723
• DOI: doi:10.1038/s41467-022-28974-6
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Ion channels
• Sach-SW: Ion channels in the nervous system
• Sach-SW: Membrane biophysics
• Sach-SW: Patch clamp
• Sach-SW: Permeation and transport
• K10plus-PPN: 1810694760

Abstract

A central question in mechanobiology is how mechanical forces acting in or on cells are transmitted to mechanically-gated PIEZO channels that convert these forces into biochemical signals. Here we examined the role of the intracellular domains of PIEZO2, which account for 25% of the channel, and demonstrate that these domains fine-tune properties such as poking and stretch-sensitivity, velocity coding and single channel conductance. Moreover, we show that the intrinsically disordered linker between the transmembrane helices twelve and thirteen (IDR5) is required for the activation of PIEZO2 by cytoskeleton-transmitted forces. The deletion of IDR5 abolishes PIEZO2-mediated inhibition of neurite outgrowth, while it only partially affected its sensitivity to cell indentation and does not alter its stretch sensitivity. Thus, we propose that PIEZO2 is a polymodal mechanosensor that detects different types of mechanical stimuli via different force transmission pathways, which highlights the importance of utilizing multiple complementary assays when investigating PIEZO function.