Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells

• Verfasst von: Hoffmann, Sabrina [VerfasserIn]
• Verfasst von: Hadji Hosseini, Babak [VerfasserIn]
• Verfasst von: Hecker, Markus [VerfasserIn]
• Verfasst von: Louban, Ilia [VerfasserIn]
• Verfasst von: Bulbuc, Nadja [VerfasserIn]
• Verfasst von: Garbi, Natalio [VerfasserIn]
• Verfasst von: Wabnitz, Guido H. [VerfasserIn]
• Verfasst von: Samstag, Yvonne [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Verfasst von: Hämmerling, Günter J. [VerfasserIn]
• Titel: Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells
• Verf.angabe: Sabrina Hoffmann, Babak H. Hosseini, Markus Hecker, Ilia Louban, Nadja Bulbuc, Natalio Garbi, Guido H. Wabnitz, Yvonne Samstag, Joachim P. Spatz, Günter J. Hämmerling
• E-Jahr: 2011
• Jahr: [30 April 2011]
• Umfang: 8 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 21.07.2022
• Titel Quelle: Enthalten in: Immunology letters
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1979
• Jahr Quelle: 2011
• Band/Heft Quelle: 136(2011), 1 vom: Apr., Seite 13-20
• ISSN Quelle: 1879-0542
• DOI: doi:10.1016/j.imlet.2010.11.005
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Antigen presentation
• Sach-SW: Atomic force microscopy
• Sach-SW: Cellular interaction forces
• Sach-SW: Intercellular adhesion molecule-1 (ICAM-1)
• K10plus-PPN: 1811294847

Abstract

T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy for quantitation of interaction forces between T-cells and APCs presenting a tolerogenic peptide derived from myelin basic protein. When T-cells were brought into contact with peptide-loaded APCs, interaction forces increased with time from about 0.5nN after 10s interaction to about 15nN after 30min. In the absence of antigen, or when ICAM-1-negative APC was used, no increase in binding forces was observed. The temporal development of interaction forces correlated with the kinetics of immune synapse formation, as determined by LFA-1 and TCR enrichment at the interface of T-cell/APC conjugates using high throughput multispectral imaging flow cytometry. Together, these results suggest that ICAM-1/LFA-1 redistribution to the contact area is mainly responsible for development of strong interaction forces. High forces will keep T-cells and APCs in tight contact, thereby providing a platform for optimal interaction between TCRs and peptide-MHC complexes.