Imaging label-free intracellular structures by localisation microscopy

• Verfasst von: Kaufmann, Rainer [VerfasserIn]
• Verfasst von: Müller, Patrick [VerfasserIn]
• Verfasst von: Hausmann, Michael [VerfasserIn]
• Verfasst von: Cremer, Christoph [VerfasserIn]
• Titel: Imaging label-free intracellular structures by localisation microscopy
• Verf.angabe: Rainer Kaufmann, Patrick Müller, Michael Hausmann, Christoph Cremer
• Jahr: 2011
• Umfang: 5 S.
• Fussnoten: Available online 28 May 2010 ; Gesehen am 25.07.2022
• Titel Quelle: Enthalten in: Micron
• Ort Quelle: New York, NY [u.a.] : Elsevier, 1969
• Jahr Quelle: 2011
• Band/Heft Quelle: 42(2011), 4, Seite 348-352
• ISSN Quelle: 1878-4291
• DOI: doi:10.1016/j.micron.2010.03.006
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Fluorescence
• Sach-SW: Localisation microscopy
• Sach-SW: Spectral Precision Distance Microscopy (SPDM)
• Sach-SW: Sub-diffraction limit microscopy
• K10plus-PPN: 1811456065

Abstract

Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200nm laterally and 600nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the λ/10 range (∼50nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions.