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Verfasst von:Keller, Andreas [VerfasserIn]   i
 Harz, Christian [VerfasserIn]   i
 Matzas, Mark [VerfasserIn]   i
 Meder, Benjamin [VerfasserIn]   i
 Katus, Hugo [VerfasserIn]   i
 Ludwig, Nicole [VerfasserIn]   i
 Fischer, Ulrike [VerfasserIn]   i
 Meese, Eckart [VerfasserIn]   i
Titel:Identification of novel SNPs in glioblastoma using targeted resequencing
Verf.angabe:Andreas Keller, Christian Harz, Mark Matzas, Benjamin Meder, Hugo A. Katus, Nicole Ludwig, Ulrike Fischer, Eckart Meese
E-Jahr:2011
Jahr:June 10, 2011
Umfang:8 S.
Fussnoten:Gesehen am 28.07.2022
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2011
Band/Heft Quelle:6(2011), 6, Artikel-ID e18158, Seite 1-8
ISSN Quelle:1932-6203
Abstract:High-throughput sequencing opens avenues to find genetic variations that may be indicative of an increased risk for certain diseases. Linking these genomic data to other “omics” approaches bears the potential to deepen our understanding of pathogenic processes at the molecular level. To detect novel single nucleotide polymorphisms (SNPs) for glioblastoma multiforme (GBM), we used a combination of specific target selection and next generation sequencing (NGS). We generated a microarray covering the exonic regions of 132 GBM associated genes to enrich target sequences in two GBM tissues and corresponding leukocytes of the patients. Enriched target genes were sequenced with Illumina and the resulting reads were mapped to the human genome. With this approach we identified over 6000 SNPs, including over 1300 SNPs located in the targeted genes. Integrating the genome-wide association study (GWAS) catalog and known disease associated SNPs, we found that several of the detected SNPs were previously associated with smoking behavior, body mass index, breast cancer and high-grade glioma. Particularly, the breast cancer associated allele of rs660118 SNP in the gene SART1 showed a near doubled frequency in glioblastoma patients, as verified in an independent control cohort by Sanger sequencing. In addition, we identified SNPs in 20 of 21 GBM associated antigens providing further evidence that genetic variations are significantly associated with the immunogenicity of antigens.
DOI:doi:10.1371/journal.pone.0018158
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0018158
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0018158
 DOI: https://doi.org/10.1371/journal.pone.0018158
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Breast cancer
 Cancers and neoplasms
 Colorectal cancer
 DNA
 Glioblastoma multiforme
 Human genomics
 Single nucleotide polymorphisms
 White blood cells
K10plus-PPN:181188153X
Verknüpfungen:→ Zeitschrift

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