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| Verfasst von: | Casati, Beatrice [VerfasserIn]  |
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| | Verdi, Joseph Peter [VerfasserIn] |
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| | Hempelmann, Alexander [VerfasserIn] |
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| | Kittel, Maximilian [VerfasserIn] |
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| | Gutierrez Klaebisch, Andrea [VerfasserIn] |
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| | Meister, Bianca [VerfasserIn] |
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| | Welker, Sybille [VerfasserIn] |
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| | Asthana, Sonal [VerfasserIn] |
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| | Di Giorgio, Salvatore [VerfasserIn] |
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| | Boskovic, Pavle [VerfasserIn] |
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| | Man, Ka-Hou [VerfasserIn] |
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| | Schopp, Meike [VerfasserIn] |
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| | Ginno, Paul Adrian [VerfasserIn] |
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| | Radlwimmer, Bernhard [VerfasserIn] |
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| | Stebbins, Erec [VerfasserIn] |
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| | Miethke, Thomas [VerfasserIn] |
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| | Papavasiliou, Nina [VerfasserIn] |
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| | Pecori, Riccardo [VerfasserIn] |
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| Titel: | Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO |
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| Verf.angabe: | Beatrice Casati, Joseph Peter Verdi, Alexander Hempelmann, Maximilian Kittel, Andrea Gutierrez Klaebisch, Bianca Meister, Sybille Welker, Sonal Asthana, Salvatore Di Giorgio, Pavle Boskovic, Ka Hou Man, Meike Schopp, Paul Adrian Ginno, Bernhard Radlwimmer, Charles Erec Stebbins, Thomas Miethke, Fotini Nina Papavasiliou & Riccardo Pecori |
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| E-Jahr: | 2022 |
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| Jahr: | 08 June 2022 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 28.07.2022 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 13(2022), Artikel-ID 3308, Seite 1-11 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | During the ongoing COVID-19 pandemic, PCR testing and antigen tests have proven critical for helping to stem the spread of its causative agent, SARS-CoV-2. However, these methods suffer from either general applicability and/or sensitivity. Moreover, the emergence of variant strains creates the need for flexibility to correctly and efficiently diagnose the presence of substrains. To address these needs we developed the diagnostic test ADESSO (Accurate Detection of Evolving SARS-CoV-2 through SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing) Optimization) which employs Cas13 to diagnose patients in 1 h without sophisticated equipment. Using an extensive panel of clinical samples, we demonstrate that ADESSO correctly identifies infected individuals at a sensitivity and specificity comparable to RT-qPCR on extracted RNA and higher than antigen tests for unextracted samples. Altogether, ADESSO is a fast, sensitive and cheap method that can be applied in a point of care setting to diagnose COVID-19 and can be quickly adjusted to detect new variants. |
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| DOI: | doi:10.1038/s41467-022-30862-y |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-022-30862-y |
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| | Volltext: https://www.nature.com/articles/s41467-022-30862-y |
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| | DOI: https://doi.org/10.1038/s41467-022-30862-y |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Biotechnology |
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| | CRISPR-Cas systems |
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| | Epidemiology |
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| | Infectious-disease diagnostics |
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| | SARS-CoV-2 |
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| K10plus-PPN: | 1811887562 |
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| Verknüpfungen: | → Zeitschrift |
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Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSO / Casati, Beatrice [VerfasserIn]; 08 June 2022 (Online-Ressource)
