Interleukin-17A and interleukin-22 production by conventional and non-conventional lymphocytes in three different end-stage lung diseases

• Verfasst von: Albrecht, Melanie [VerfasserIn]
• Verfasst von: Halle, Olga [VerfasserIn]
• Verfasst von: Gaedcke, Svenja [VerfasserIn]
• Verfasst von: Pallenberg, Sophia T [VerfasserIn]
• Verfasst von: Camargo Neumann, Julia [VerfasserIn]
• Verfasst von: Witt, Marius [VerfasserIn]
• Verfasst von: Roediger, Johanna [VerfasserIn]
• Verfasst von: Schumacher, Marina [VerfasserIn]
• Verfasst von: Jirmo, Adan Chari [VerfasserIn]
• Verfasst von: Warnecke, Gregor [VerfasserIn]
• Verfasst von: Jonigk, Danny [VerfasserIn]
• Verfasst von: Braubach, Peter [VerfasserIn]
• Verfasst von: DeLuca, David [VerfasserIn]
• Verfasst von: Hansen, Gesine [VerfasserIn]
• Verfasst von: Dittrich, Anna-Maria [VerfasserIn]
• Titel: Interleukin-17A and interleukin-22 production by conventional and non-conventional lymphocytes in three different end-stage lung diseases
• Verf.angabe: Melanie Albrecht, Olga Halle, Svenja Gaedcke, Sophia T Pallenberg, Julia Camargo Neumann, Marius Witt, Johanna Roediger, Marina Schumacher, Adan Chari Jirmo, Gregor Warnecke, Danny Jonigk, Peter Braubach, David DeLuca, Gesine Hansen & Anna-Maria Dittrich
• E-Jahr: 2022
• Jahr: 16 June 2022
• Umfang: 18 S.
• Fussnoten: Gesehen am 29.07.2022
• Titel Quelle: Enthalten in: Clinical & Translational Immunology
• Ort Quelle: Hoboken, NJ : Wiley, 2012
• Jahr Quelle: 2022
• Band/Heft Quelle: 11(2022), 6, Artikel-ID e1398, Seite 1-18
• ISSN Quelle: 2050-0068
• DOI: doi:10.1002/cti2.1398
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cystic fibrosis
• Sach-SW: end-stage lung disease
• Sach-SW: IL-17
• Sach-SW: innate lymphoid cells
• K10plus-PPN: 1811982255

Abstract

Objectives The contribution of adaptive vs. innate lymphocytes to IL-17A and IL-22 secretion at the end stage of chronic lung diseases remains largely unexplored. In order to uncover tissue- and disease-specific secretion patterns, we compared production patterns of IL-17A and IL-22 in three different human end-stage lung disease entities. Methods Production of IL-17A, IL-22 and associated cytokines was assessed in supernatants of re-stimulated lymphocytes by multiplex assays and multicolour flow cytometry of conventional T cells, iNKT cells, γδ T cells and innate lymphoid cells in bronchial lymph node and lung tissue from patients with emphysema (n = 19), idiopathic pulmonary fibrosis (n = 14) and cystic fibrosis (n = 23), as well as lung donors (n = 17). Results We detected secretion of IL-17A and IL-22 by CD4+ T cells, CD8+ T cells, innate lymphoid cells, γδ T cells and iNKT cells in all end-stage lung disease entities. Our analyses revealed disease-specific contributions of individual lymphocyte subpopulations to cytokine secretion patterns. We furthermore found the high levels of microbial detection in CF samples to associate with a more pronounced IL-17A signature upon antigen-specific and unspecific re-stimulation compared to other disease entities and lung donors. Conclusion Our results show that both adaptive and innate lymphocyte populations contribute to IL-17A-dependent pathologies in different end-stage lung disease entities, where they establish an IL-17A-rich microenvironment. Microbial colonisation patterns and cytokine secretion upon microbial re-stimulation suggest that pathogens drive IL-17A secretion patterns in end-stage lung disease.