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Verfasst von:Harenberg, Job [VerfasserIn]   i
 Hetjens, Svetlana [VerfasserIn]   i
 Krämer, Roland [VerfasserIn]   i
 Giese, Christina [VerfasserIn]   i
 Weiß, Christel [VerfasserIn]   i
Titel:Determination of an international sensitivity index of thromboplastin reagents using a WHO thromboplastin as calibrator for plasma spiked with rivaroxaban
Verf.angabe:Job Harenberg, Svetlana Marx, Roland Krämer, Christina Giese and Christel Weiss
Jahr:2011
Umfang:5 S.
Fussnoten:Gesehen am 03.08.2022
Titel Quelle:Enthalten in: Blood coagulation & fibrinolysis
Ort Quelle:Hagerstown, Md. : Lippincott, Williams & Wilkins, 1990
Jahr Quelle:2011
Band/Heft Quelle:22(2011), 8, Seite 637-641
ISSN Quelle:1473-5733
Abstract:Rivaroxaban and other direct factor Xa inhibitors are used at fixed doses without drug monitoring and dose adjustment. Patients may require determination of the anticoagulant effect during treatment. The aim of this study was to develop a method to reduce the differences between thromboplastin reagents and coagulation analysers for determination of the anticoagulant effect of rivaroxaban in human plasma. Purity of rivaroxaban extracted from commercially available drug was confirmed by mass spectrometry, elemental analysis and 1H-NMR spectroscopy. Coagulation times of pooled human plasma spiked with 50-900 ng/ml rivaroxaban were analysed. Thromboplastin reagents, WHO RBT/90, Innovin, RecombiPlasTin 2G, STA Neoplastin Plus, Technoclot PT Plus and Thromborel S, the manual Kolle-Hook method and the KC10 analyser were used. An international sensitivity index (ISI) was determined for each reagent and coagulation method using the RBT/90 thromboplastin reagent as reference. The orthogonal, used for the determination of the ISI of coumarin plasmas, and ordinary regression analyses were compared. The results showed than increasing concentrations of rivaroxaban prolonged coagulation values of all thromboplastin assays linearly (r2 = 0.96 and r2 = 0.99, respectively). The coefficient of variation between the slopes of the dilution curves and the ratios of the thromboplastin reagents were reduced using the international normalized ratio (INR) and ISI calculated for rivaroxaban. The ISIs of the thromboplastin reagents ranged from 0.73 to 1.67 as compared with the WHO reagent using the manual technique. The coefficient of variations between the thromboplastin reagents comparing the orthogonal and the ordinary regression analysis were 6.8 versus 3.7% (Kolle-Hook method, P = 0.0011) and 8.5 versus 4.8% (KC10 method, P < 0.0001). Using ISI for vitamin-K antagonist and rivaroxaban, the INRs for the rivaroxaban-containing samples were significantly different for one of five commercial thromboplastin reagents. In conclusion, the determination of an ISI for rivaroxaban using a WHO thromboplastin reagent is required for commercial thromboplastin reagents. The manual Kolle-Hook method and an ordinary linear regression analysis should be adopted.
DOI:doi:10.1097/MBC.0b013e328349f1d6
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1097/MBC.0b013e328349f1d6
 Volltext: https://journals.lww.com/bloodcoagulation/Fulltext/2011/12000/Determination_of_an_international_sensitivity.2.aspx
 DOI: https://doi.org/10.1097/MBC.0b013e328349f1d6
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1813079218
Verknüpfungen:→ Zeitschrift

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