PINCH proteins regulate cardiac contractility by modulating integrin-linked kinase-protein kinase B signaling

• Verfasst von: Meder, Benjamin [VerfasserIn]
• Verfasst von: Huttner, Inken G. [VerfasserIn]
• Verfasst von: Sedaghat-Hamedani, Farbod [VerfasserIn]
• Verfasst von: Just, Steffen [VerfasserIn]
• Verfasst von: Dahme, Tillman [VerfasserIn]
• Verfasst von: Frese, Karen S. [VerfasserIn]
• Verfasst von: Heilmeier, Britta [VerfasserIn]
• Verfasst von: Köhler, Doreen [VerfasserIn]
• Verfasst von: Kloos, Wanda [VerfasserIn]
• Verfasst von: Rudloff, Jessica [VerfasserIn]
• Verfasst von: Marquart, Sabine [VerfasserIn]
• Verfasst von: Katus, Hugo [VerfasserIn]
• Verfasst von: Rottbauer, Wolfgang [VerfasserIn]
• Titel: PINCH proteins regulate cardiac contractility by modulating integrin-linked kinase-protein kinase B signaling
• Verf.angabe: Benjamin Meder, Inken G. Huttner, Farbod Sedaghat-Hamedani, Steffen Just, Tillman Dahme, Karen S. Frese, Britta Vogel, Doreen Köhler, Wanda Kloos, Jessica Rudloff, Sabine Marquart, Hugo A. Katus, and Wolfgang Rottbauer
• E-Jahr: 2011
• Jahr: 27 July 2011
• Umfang: 10 S.
• Fussnoten: Gesehen am 15.08.2022
• Titel Quelle: Enthalten in: Molecular and cellular biology
• Ort Quelle: Washington, DC : Soc., 1981
• Jahr Quelle: 2011
• Band/Heft Quelle: 31(2011), 16, Seite 3424-3435
• ISSN Quelle: 1098-5549
• DOI: doi:10.1128/MCB.05269-11
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1814315306

Abstract

Integrin-linked kinase (ILK) is an essential component of the cardiac mechanical stretch sensor and is bound in a protein complex with parvin and PINCH proteins, the so-called ILK-PINCH-parvin (IPP) complex. We have recently shown that inactivation of ILK or β-parvin activity leads to heart failure in zebrafish via reduced protein kinase B (PKB/Akt) activation. Here, we show that PINCH proteins localize at sarcomeric Z disks and costameres in the zebrafish heart and skeletal muscle. To investigate the in vivo role of PINCH proteins for IPP complex stability and PKB signaling within the vertebrate heart, we inactivated PINCH1 and PINCH2 in zebrafish. Inactivation of either PINCH isoform independently leads to instability of ILK, loss of stretch-responsive anf and vegf expression, and progressive heart failure. The predominant cause of heart failure in PINCH morphants seems to be loss of PKB activity, since PKB phosphorylation at serine 473 is significantly reduced in PINCH-deficient hearts and overexpression of constitutively active PKB reconstitutes cardiac function in PINCH morphants. These findings highlight the essential function of PINCH proteins in controlling cardiac contractility by granting IPP/PKB-mediated signaling.