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Verfasst von:Hu, Xiaoli [VerfasserIn]   i
 Agarwal, Nitin [VerfasserIn]   i
 Zhang, Ming-Dong [VerfasserIn]   i
 Ernfors, Patrik [VerfasserIn]   i
 Kuner, Rohini [VerfasserIn]   i
 Nyengaard, Jens Randel [VerfasserIn]   i
 Karlsson, Pall [VerfasserIn]   i
Titel:Identification and quantification of nociceptive Schwann cells in mice with and without Streptozotocin-induced diabetes
Verf.angabe:Xiaoli Hu, Nitin Agarwal, Ming-Dong Zhang, Patrik Ernfors, Rohini Kuner, Jens Randel Nyengaard, Pall Karlsson
E-Jahr:2022
Jahr:6 June 2022
Umfang:10 S.
Fussnoten:Gesehen am 23.08.2022
Titel Quelle:Enthalten in: Journal of chemical neuroanatomy
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1991
Jahr Quelle:2022
Band/Heft Quelle:123(2022) vom: Sept., Artikel-ID 102118, Seite 1-10
ISSN Quelle:1873-6300
Abstract:Specialized cutaneous Schwann cells (SCs), termed nociceptive SCs, were recently discovered. Their function is not fully understood, but they are believed not only to support peripheral axons in mouse skin by forming a mesh-like neural-glio networking structure in subepidermal area, but also contributing to transduction of mechanical sensation and neuropathic pain. Diabetic neuropathy (DPN) is one of the most common complication of diabetes, however, the mechanisms behind painful and painless DPN remain unclear. Using a mouse model of DPN, we want to investigate if there are quantitative differences in nociceptive SC density between the condition of hyperglycemia-induced sensory abnormalities and control condition and at which stage in the disease the damage occurs. Here, we developed a set of counting rules for nociceptive SCs based on immunofluorescent staining, and applied the method to quantify the density of nociceptive SCs in control mice (n = 10), mice with nociceptive hypersensitivity at early diabetic stage (n = 5), and mice with sensory hyposensitivity at late diabetic stage (n = 5) in the Streptozotocin (STZ) model of type 1 diabetes. Nociceptive SCs were identified as S100+/Sox10+/DAPI+ cells abutting to peripheral nerves, with the somas located within 25 µm depth in the subepidermal area and outside glands and large fiber bundles. Hypersensitive diabetic mice had decreased nociceptive SC density, despite having normal epidermal nerve fiber density, compared with age-matched control mice (P = 0.023). In contrast, there was a reduction in intraepidermal nerve fiber density but no difference in nociceptive SC density between hyposensitive diabetic mice and the age-matched control mice. This study provides a detailed description of how to identify and quantify nociceptive SC and demonstrates that nociceptive SC density declines before nerve fiber deterioration, which supports previous observations that nociceptive SCs are critical for maintenance of cutaneous sensory nerves.
DOI:doi:10.1016/j.jchemneu.2022.102118
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.jchemneu.2022.102118
 Volltext: https://www.sciencedirect.com/science/article/pii/S0891061822000485
 DOI: https://doi.org/10.1016/j.jchemneu.2022.102118
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Diabetic polyneuropathy
 Hyperglycemia
 Intraepidermal nerve fiber
 Nociceptive Schwann cell
 Type 1 diabetes
K10plus-PPN:1814981438
Verknüpfungen:→ Zeitschrift

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