Heterogeneous fates of simultaneously-born neurons in the cortical ventricular zone

• Verfasst von: Magrinelli, Elia [VerfasserIn]
• Verfasst von: Baumann, Natalia [VerfasserIn]
• Verfasst von: Wagener, Jan Robin [VerfasserIn]
• Verfasst von: Glangetas, Christelle [VerfasserIn]
• Verfasst von: Bellone, Camilla [VerfasserIn]
• Verfasst von: Jabaudon, Denis [VerfasserIn]
• Verfasst von: Klingler, Esther [VerfasserIn]
• Titel: Heterogeneous fates of simultaneously-born neurons in the cortical ventricular zone
• Verf.angabe: Elia Magrinelli, Natalia Baumann, Robin Jan Wagener, Christelle Glangetas, Camilla Bellone, Denis Jabaudon & Esther Klingler
• E-Jahr: 2022
• Jahr: 11 April 2022
• Umfang: 11 S.
• Fussnoten: Gesehen am 07.09.2022
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2022
• Band/Heft Quelle: 12(2022), Artikel-ID 6022, Seite 1-11
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/s41598-022-09740-6
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cell fate and cell lineage
• Sach-SW: Cell type diversity
• Sach-SW: Developmental neurogenesis
• Sach-SW: Lamination
• Sach-SW: Neural progenitors
• Sach-SW: Neuronal development
• K10plus-PPN: 1815986409

Abstract

Neocortical excitatory neurons belong to diverse cell types, which can be distinguished by their dates of birth, laminar location, connectivity, and molecular identities. During embryogenesis, apical progenitors (APs) located in the ventricular zone first give birth to deep-layer neurons, and next to superficial-layer neurons. While the overall sequential construction of neocortical layers is well-established, whether APs produce multiple neuron types at single time points of corticogenesis is unknown. To address this question, here we used FlashTag to fate-map simultaneously-born (i.e. isochronic) cohorts of AP daughter neurons at successive stages of corticogenesis. We reveal that early in corticogenesis, isochronic neurons differentiate into heterogeneous laminar, hodological and molecular cell types. Later on, instead, simultaneously-born neurons have more homogeneous fates. Using single-cell gene expression analyses, we identify an early postmitotic surge in the molecular heterogeneity of nascent neurons during which some early-born neurons initiate and partially execute late-born neuron transcriptional programs. Together, these findings suggest that as corticogenesis unfolds, mechanisms allowing increased homogeneity in neuronal output are progressively implemented, resulting in progressively more predictable neuronal identities.