| |  Online-Ressource |
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| Verfasst von: | Thrun, Anna [VerfasserIn]  |
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| | Garzia, Aitor [VerfasserIn] |
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| | Kigoshi-Tansho, Yu [VerfasserIn] |
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| | Patil, Pratik Rajendra [VerfasserIn] |
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| | Umbaugh, Charles Samuel [VerfasserIn] |
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| | Dallinger, Teresa [VerfasserIn] |
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| | Liu, Jia [VerfasserIn] |
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| | Kreger, Sylvia [VerfasserIn] |
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| | Patrizi, Annarita [VerfasserIn] |
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| | Cox, Gregory A. [VerfasserIn] |
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| | Tuschl, Thomas [VerfasserIn] |
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| | Joazeiro, Claudio A. P. [VerfasserIn] |
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| Titel: | Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing |
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| Verf.angabe: | Anna Thrun, Aitor Garzia, Yu Kigoshi-Tansho, Pratik R. Patil, Charles S. Umbaugh, Teresa Dallinger, Jia Liu, Sylvia Kreger, Annarita Patrizi, Gregory A. Cox, Thomas Tuschl, and Claudio A.P. Joazeiro |
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| E-Jahr: | 2021 |
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| Jahr: | April 27, 2021 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 14.09.2022 |
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| Titel Quelle: | Enthalten in: Molecular cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 81(2021), 10, Seite 2112-2122.e7 |
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| ISSN Quelle: | 1097-4164 |
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| Abstract: | Incompletely synthesized nascent chains obstructing large ribosomal subunits are targeted for degradation by ribosome-associated quality control (RQC). In bacterial RQC, RqcH marks the nascent chains with C-terminal alanine (Ala) tails that are directly recognized by proteasome-like proteases, whereas in eukaryotes, RqcH orthologs (Rqc2/NEMF [nuclear export mediator factor]) assist the Ltn1/Listerin E3 ligase in nascent chain ubiquitylation. Here, we study RQC-mediated proteolytic targeting of ribosome stalling products in mammalian cells. We show that mammalian NEMF has an additional, Listerin-independent proteolytic role, which, as in bacteria, is mediated by tRNA-Ala binding and Ala tailing. However, in mammalian cells Ala tails signal proteolysis indirectly, through a pathway that recognizes C-terminal degrons; we identify the CRL2KLHDC10 E3 ligase complex and the novel C-end rule E3, Pirh2/Rchy1, as bona fide RQC pathway components that directly bind to Ala-tailed ribosome stalling products and target them for degradation. As Listerin mutation causes neurodegeneration in mice, functionally redundant E3s may likewise be implicated in molecular mechanisms of neurodegeneration. |
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| DOI: | doi:10.1016/j.molcel.2021.03.004 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.molcel.2021.03.004 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1097276521001726 |
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| | DOI: https://doi.org/10.1016/j.molcel.2021.03.004 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Alanine-tail |
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| | C-end rule |
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| | KLHDC10 |
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| | NEMF |
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| | Pirh2 |
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| | Rchy1 |
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| | ribosome-associated quality control |
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| | RQC |
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| | Rqc2 |
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| | RqcH |
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| K10plus-PPN: | 1816652288 |
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| Verknüpfungen: | → Zeitschrift |
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Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing / Thrun, Anna [VerfasserIn]; April 27, 2021 (Online-Ressource)
