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Verfasst von:Ruef, Peter [VerfasserIn]   i
 Ehrhard, M. [VerfasserIn]   i
 Frommhold, David [VerfasserIn]   i
 Koch, L. [VerfasserIn]   i
 Fritzsching, Benedikt [VerfasserIn]   i
 Pöschl, Johannes [VerfasserIn]   i
Titel:Lipid A decreases human erythrocytes deformability by increasing intracellular Ca2+
Titelzusatz:Effects of verapamil, staurosporine and the rho-kinase inhibitor Y-27632
Verf.angabe:P. Ruef, M. Ehrhard, D. Frommhold, L. Koch, B. Fritzsching and J. Poeschl
Jahr:2011
Umfang:8 S.
Fussnoten:Gesehen am 21.09.2022 ; Im Titel ist der Ausdruck "2+" hochgestellt
Titel Quelle:Enthalten in: Clinical hemorheology and microcirculation
Ort Quelle:Amsterdam [u.a.] : IOS Press, 1997
Jahr Quelle:2011
Band/Heft Quelle:49(2011), 1/4, Seite 315-322
ISSN Quelle:1875-8622
Abstract:There are several reports demonstrating an involvement of bacterial toxins in the rigidity of red blood cells (RBC). The present study investigates the influence of E. coli F-583-Rd lipid A on RBC deformability under mechanical shear stress. Verapamil (Ca2+ channel inhibitor), staurosporine (protein kinase inhibitor) and Y-27632 (rho-kinase inhibitor) were used to modify the effect of lipid A on RBC deformability. We also determined if E. coli F-583-Rd Lipid A could induce an increase of intracellular Ca2+ concentration. For the deformation measurements RBC (10 adult donors) were incubated with E. coli F-583-Rd lipid A (100μg/ml) and also co-incubated with either verapamil (10-7 mol/l), staurosporine (10-7 mol/l) or Y-27632 (10-7 mol/l). The deformation of the RBC under different shear stresses (0.6-60 Pa) was measured by a shear stress diffractometer (Rheodyne SSD). Intracellular Ca2+ was determinded by flow cytometry in RBC incubated with Lipid A and labeled with fluorescent Fluo-4/AM which binds intracellular Ca2+ with high affinity resulting in enhanced green fluorescence intensity. At increasing shear stresses Lipid A induced a significantly lower elongation. Co-incubation of the erythrocytes with verapamil or staurosporine inhibited lipid A induced decrease in elongation while Y-27632 had no effect. Verapamil, Staurosporine and Y-27632 did not influence the elongation response of the cells under control conditions. Lipid A induced a marked increase in fluorescence Fluo-4/AM indicating increased intracellular Ca2+. These results suggest that E. coli F-583-Rd lipid A is able to influence red blood cell rigidity by a rapid and significant increase of intracellular Ca2+ concentration. Verapamil and staurosporine abolished the decrease in deformability of Lipid A incubated RBC.
DOI:doi:10.3233/CH-2011-1482
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://dx.doi.org/10.3233/CH-2011-1482
 Volltext: http://www.redi-bw.de/db/ebsco.php/search.ebscohost.com/login.aspx%3fdirect%3dtrue%26db%3da9h%26AN%3d70081430%26site%3de ...
 DOI: https://doi.org/10.3233/CH-2011-1482
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:BACTERIAL toxins
 Ca$^{2+}$
 deformability
 ERYTHROCYTE deformability
 FLOW cytometry
 INTRACELLULAR calcium
 lipid A
 PROTEIN kinases
 Red blood cell
 rho-kinase-inhibitor
 sepsis
 staurosporine
 verapamil
 VERAPAMIL
K10plus-PPN:1817208950
Verknüpfungen:→ Zeitschrift

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