| Online-Ressource |
Verfasst von: | Ruef, Peter [VerfasserIn]  |
| Ehrhard, M. [VerfasserIn]  |
| Frommhold, David [VerfasserIn]  |
| Koch, L. [VerfasserIn]  |
| Fritzsching, Benedikt [VerfasserIn]  |
| Pöschl, Johannes [VerfasserIn]  |
Titel: | Lipid A decreases human erythrocytes deformability by increasing intracellular Ca2+ |
Titelzusatz: | Effects of verapamil, staurosporine and the rho-kinase inhibitor Y-27632 |
Verf.angabe: | P. Ruef, M. Ehrhard, D. Frommhold, L. Koch, B. Fritzsching and J. Poeschl |
Jahr: | 2011 |
Umfang: | 8 S. |
Fussnoten: | Gesehen am 21.09.2022 ; Im Titel ist der Ausdruck "2+" hochgestellt |
Titel Quelle: | Enthalten in: Clinical hemorheology and microcirculation |
Ort Quelle: | Amsterdam [u.a.] : IOS Press, 1997 |
Jahr Quelle: | 2011 |
Band/Heft Quelle: | 49(2011), 1/4, Seite 315-322 |
ISSN Quelle: | 1875-8622 |
Abstract: | There are several reports demonstrating an involvement of bacterial toxins in the rigidity of red blood cells (RBC). The present study investigates the influence of E. coli F-583-Rd lipid A on RBC deformability under mechanical shear stress. Verapamil (Ca2+ channel inhibitor), staurosporine (protein kinase inhibitor) and Y-27632 (rho-kinase inhibitor) were used to modify the effect of lipid A on RBC deformability. We also determined if E. coli F-583-Rd Lipid A could induce an increase of intracellular Ca2+ concentration. For the deformation measurements RBC (10 adult donors) were incubated with E. coli F-583-Rd lipid A (100μg/ml) and also co-incubated with either verapamil (10-7 mol/l), staurosporine (10-7 mol/l) or Y-27632 (10-7 mol/l). The deformation of the RBC under different shear stresses (0.6-60 Pa) was measured by a shear stress diffractometer (Rheodyne SSD). Intracellular Ca2+ was determinded by flow cytometry in RBC incubated with Lipid A and labeled with fluorescent Fluo-4/AM which binds intracellular Ca2+ with high affinity resulting in enhanced green fluorescence intensity. At increasing shear stresses Lipid A induced a significantly lower elongation. Co-incubation of the erythrocytes with verapamil or staurosporine inhibited lipid A induced decrease in elongation while Y-27632 had no effect. Verapamil, Staurosporine and Y-27632 did not influence the elongation response of the cells under control conditions. Lipid A induced a marked increase in fluorescence Fluo-4/AM indicating increased intracellular Ca2+. These results suggest that E. coli F-583-Rd lipid A is able to influence red blood cell rigidity by a rapid and significant increase of intracellular Ca2+ concentration. Verapamil and staurosporine abolished the decrease in deformability of Lipid A incubated RBC. |
DOI: | doi:10.3233/CH-2011-1482 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.3233/CH-2011-1482 |
| Volltext: http://www.redi-bw.de/db/ebsco.php/search.ebscohost.com/login.aspx%3fdirect%3dtrue%26db%3da9h%26AN%3d70081430%26site%3de ... |
| DOI: https://doi.org/10.3233/CH-2011-1482 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | BACTERIAL toxins |
| Ca$^{2+}$ |
| deformability |
| ERYTHROCYTE deformability |
| FLOW cytometry |
| INTRACELLULAR calcium |
| lipid A |
| PROTEIN kinases |
| Red blood cell |
| rho-kinase-inhibitor |
| sepsis |
| staurosporine |
| verapamil |
| VERAPAMIL |
K10plus-PPN: | 1817208950 |
Verknüpfungen: | → Zeitschrift |
Lipid A decreases human erythrocytes deformability by increasing intracellular Ca2+ / Ruef, Peter [VerfasserIn]; 2011 (Online-Ressource)