Navigation überspringen
Universitätsbibliothek Heidelberg
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Zhong, Rujia [VerfasserIn]   i
 Zhang, Feng [VerfasserIn]   i
 Yang, Zhen [VerfasserIn]   i
 Li, Yingrui [VerfasserIn]   i
 Xu, Qiang [VerfasserIn]   i
 Lan, Huan [VerfasserIn]   i
 Lang, Siegfried [VerfasserIn]   i
 Cyganek, Lukas [VerfasserIn]   i
 Burgermeister, Elke [VerfasserIn]   i
 El-Battrawy, Ibrahim [VerfasserIn]   i
 Zhou, Xiao-Bo [VerfasserIn]   i
 Akın, Ibrahim [VerfasserIn]   i
 Borggrefe, Martin [VerfasserIn]   i
Titel:Regulation of ion channel function in human-induced pluripotent stem cell-derived cardiomyocytes by cancer cell secretion through DNA methylation
Verf.angabe:Rujia Zhong, Feng Zhang, Zhen Yang, Yingrui Li, Qiang Xu, Huan Lan, Siegfried Lang, Lukas Cyganek, Elke Burgermeister, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin and Martin Borggrefe
E-Jahr:2022
Jahr:21 February 2022
Umfang:13 S.
Fussnoten:Gesehen am 27.09.2022
Titel Quelle:Enthalten in: Frontiers in Cardiovascular Medicine
Ort Quelle:Lausanne : Frontiers Media, 2014
Jahr Quelle:2022
Band/Heft Quelle:9(2022) vom: Feb., Artikel-ID 839104, Seite 1-13
ISSN Quelle:2297-055X
Abstract:Background - Cardiac dysfunctions including arrhythmias appear frequently in patients with cancers, which are expected to be caused mainly by cardiotoxic effects of chemotherapy. Experimental studies investigating the effects of cancer cell secretion without chemotherapy on ion channel functions in human cardiomyocytes are still lacking. Methods - The human-induced pluripotent stem cell-derived cardiomyocytes hiPSC-CMs generated from three healthy donors were treated with GI cancer (AGS and SW480 cells) medium for 48 hours. The qPCR, patch-clamp, western blotting, immunostaining, dot blotting, bisulfite sequence and overexpression of the ten-eleven translocation (TET) enzyme were performed for the study. Results - After treated with cancer cell secretion, the maximum depolarization velocity and the action potential amplitude were reduced, the action potential duration prolonged, peak Na+ current and the transient outward current were decreased, late Na+ and the slowly activating delayed rectifier K+ current were increased. Changes of mRNA and protein level of respective channels were detected along with altered DNA methylation level in CpG island in the promoter regions of ion channel genes and increased protein levels of DNA methyltransferases. PI3K inhibitor attenuated and TGF-β mimicked the effects of cancer cell secretion. Conclusions - Gastrointestinal cancer cell secretion could induce ion channel dysfunction, which may contribute to occurrence of arrhythmias in cancer patients. The ion channel dysfunctions could result from DNA methylation of ion channel genes via activation of TGF-β/PI3K signaling. This study may provide new insights into pathogenesis of cardiomyopathy in cancer patients.
DOI:doi:10.3389/fcvm.2022.839104
URL:kostenfrei: Volltext: https://doi.org/10.3389/fcvm.2022.839104
 kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fcvm.2022.839104/full
 DOI: https://doi.org/10.3389/fcvm.2022.839104
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1817563084
Verknüpfungen:→ Zeitschrift
 
 
Lokale URL UB: Zum Volltext

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68967613   QR-Code
zum Seitenanfang