| Online-Ressource |
Verfasst von: | Poetz, Fabian [VerfasserIn]  |
| Corbo, Joshua [VerfasserIn]  |
| Levdansky, Yevgen [VerfasserIn]  |
| Spiegelhalter, Alexander [VerfasserIn]  |
| Lindner, Doris [VerfasserIn]  |
| Magg, Vera [VerfasserIn]  |
| Lebedeva, Svetlana [VerfasserIn]  |
| Schweiggert, Jörg [VerfasserIn]  |
| Schott, Johanna [VerfasserIn]  |
| Valkov, Eugene [VerfasserIn]  |
| Stoecklin, Georg [VerfasserIn]  |
Titel: | RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation |
Verf.angabe: | Fabian Poetz, Joshua Corbo, Yevgen Levdansky, Alexander Spiegelhalter, Doris Lindner, Vera Magg, Svetlana Lebedeva, Jörg Schweiggert, Johanna Schott, Eugene Valkov & Georg Stoecklin |
E-Jahr: | 2021 |
Jahr: | 09 December 2021 |
Umfang: | 19 S. |
Fussnoten: | Gesehen am 27.09.2022 |
Titel Quelle: | Enthalten in: Nature Communications |
Ort Quelle: | [London] : Springer Nature, 2010 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | 12(2021), Artikel-ID 7175, Seite 1-19 |
ISSN Quelle: | 2041-1723 |
Abstract: | The CCR4-NOT complex acts as a central player in the control of mRNA turnover and mediates accelerated mRNA degradation upon HDAC inhibition. Here, we explored acetylation-induced changes in the composition of the CCR4-NOT complex by purification of the endogenously tagged scaffold subunit NOT1 and identified RNF219 as an acetylation-regulated cofactor. We demonstrate that RNF219 is an active RING-type E3 ligase which stably associates with CCR4-NOT via NOT9 through a short linear motif (SLiM) embedded within the C-terminal low-complexity region of RNF219. By using a reconstituted six-subunit human CCR4-NOT complex, we demonstrate that RNF219 inhibits deadenylation through the direct interaction of the α-helical SLiM with the NOT9 module. Transcriptome-wide mRNA half-life measurements reveal that RNF219 attenuates global mRNA turnover in cells, with differential requirement of its RING domain. Our results establish RNF219 as an inhibitor of CCR4-NOT-mediated deadenylation, whose loss upon HDAC inhibition contributes to accelerated mRNA turnover. |
DOI: | doi:10.1038/s41467-021-27471-6 |
URL: | kostenfrei: Volltext: https://doi.org/10.1038/s41467-021-27471-6 |
| kostenfrei: Volltext: https://www.nature.com/articles/s41467-021-27471-6 |
| DOI: https://doi.org/10.1038/s41467-021-27471-6 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | RNA |
| RNA decay |
K10plus-PPN: | 1817582186 |
Verknüpfungen: | → Zeitschrift |
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Lokale URL UB: | Zum Volltext |
RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation / Poetz, Fabian [VerfasserIn]; 09 December 2021 (Online-Ressource)