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Verfasst von:Poetz, Fabian [VerfasserIn]   i
 Corbo, Joshua [VerfasserIn]   i
 Levdansky, Yevgen [VerfasserIn]   i
 Spiegelhalter, Alexander [VerfasserIn]   i
 Lindner, Doris [VerfasserIn]   i
 Magg, Vera [VerfasserIn]   i
 Lebedeva, Svetlana [VerfasserIn]   i
 Schweiggert, Jörg [VerfasserIn]   i
 Schott, Johanna [VerfasserIn]   i
 Valkov, Eugene [VerfasserIn]   i
 Stoecklin, Georg [VerfasserIn]   i
Titel:RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation
Verf.angabe:Fabian Poetz, Joshua Corbo, Yevgen Levdansky, Alexander Spiegelhalter, Doris Lindner, Vera Magg, Svetlana Lebedeva, Jörg Schweiggert, Johanna Schott, Eugene Valkov & Georg Stoecklin
E-Jahr:2021
Jahr:09 December 2021
Umfang:19 S.
Fussnoten:Gesehen am 27.09.2022
Titel Quelle:Enthalten in: Nature Communications
Ort Quelle:[London] : Springer Nature, 2010
Jahr Quelle:2021
Band/Heft Quelle:12(2021), Artikel-ID 7175, Seite 1-19
ISSN Quelle:2041-1723
Abstract:The CCR4-NOT complex acts as a central player in the control of mRNA turnover and mediates accelerated mRNA degradation upon HDAC inhibition. Here, we explored acetylation-induced changes in the composition of the CCR4-NOT complex by purification of the endogenously tagged scaffold subunit NOT1 and identified RNF219 as an acetylation-regulated cofactor. We demonstrate that RNF219 is an active RING-type E3 ligase which stably associates with CCR4-NOT via NOT9 through a short linear motif (SLiM) embedded within the C-terminal low-complexity region of RNF219. By using a reconstituted six-subunit human CCR4-NOT complex, we demonstrate that RNF219 inhibits deadenylation through the direct interaction of the α-helical SLiM with the NOT9 module. Transcriptome-wide mRNA half-life measurements reveal that RNF219 attenuates global mRNA turnover in cells, with differential requirement of its RING domain. Our results establish RNF219 as an inhibitor of CCR4-NOT-mediated deadenylation, whose loss upon HDAC inhibition contributes to accelerated mRNA turnover.
DOI:doi:10.1038/s41467-021-27471-6
URL:kostenfrei: Volltext: https://doi.org/10.1038/s41467-021-27471-6
 kostenfrei: Volltext: https://www.nature.com/articles/s41467-021-27471-6
 DOI: https://doi.org/10.1038/s41467-021-27471-6
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:RNA
 RNA decay
K10plus-PPN:1817582186
Verknüpfungen:→ Zeitschrift
 
 
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