| |  Online-Ressource |
|---|
| Verfasst von: | Poetz, Fabian [VerfasserIn]  |
|---|
| | Corbo, Joshua [VerfasserIn] |
|---|
| | Levdansky, Yevgen [VerfasserIn] |
|---|
| | Spiegelhalter, Alexander [VerfasserIn] |
|---|
| | Lindner, Doris [VerfasserIn] |
|---|
| | Magg, Vera [VerfasserIn] |
|---|
| | Lebedeva, Svetlana [VerfasserIn] |
|---|
| | Schweiggert, Jörg [VerfasserIn] |
|---|
| | Schott, Johanna [VerfasserIn] |
|---|
| | Valkov, Eugene [VerfasserIn] |
|---|
| | Stoecklin, Georg [VerfasserIn] |
|---|
| Titel: | RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation |
|---|
| Verf.angabe: | Fabian Poetz, Joshua Corbo, Yevgen Levdansky, Alexander Spiegelhalter, Doris Lindner, Vera Magg, Svetlana Lebedeva, Jörg Schweiggert, Johanna Schott, Eugene Valkov & Georg Stoecklin |
|---|
| E-Jahr: | 2021 |
|---|
| Jahr: | 09 December 2021 |
|---|
| Umfang: | 19 S. |
|---|
| Fussnoten: | Gesehen am 27.09.2022 |
|---|
| Titel Quelle: | Enthalten in: Nature Communications |
|---|
| Ort Quelle: | [London] : Springer Nature, 2010 |
|---|
| Jahr Quelle: | 2021 |
|---|
| Band/Heft Quelle: | 12(2021), Artikel-ID 7175, Seite 1-19 |
|---|
| ISSN Quelle: | 2041-1723 |
|---|
| Abstract: | The CCR4-NOT complex acts as a central player in the control of mRNA turnover and mediates accelerated mRNA degradation upon HDAC inhibition. Here, we explored acetylation-induced changes in the composition of the CCR4-NOT complex by purification of the endogenously tagged scaffold subunit NOT1 and identified RNF219 as an acetylation-regulated cofactor. We demonstrate that RNF219 is an active RING-type E3 ligase which stably associates with CCR4-NOT via NOT9 through a short linear motif (SLiM) embedded within the C-terminal low-complexity region of RNF219. By using a reconstituted six-subunit human CCR4-NOT complex, we demonstrate that RNF219 inhibits deadenylation through the direct interaction of the α-helical SLiM with the NOT9 module. Transcriptome-wide mRNA half-life measurements reveal that RNF219 attenuates global mRNA turnover in cells, with differential requirement of its RING domain. Our results establish RNF219 as an inhibitor of CCR4-NOT-mediated deadenylation, whose loss upon HDAC inhibition contributes to accelerated mRNA turnover. |
|---|
| DOI: | doi:10.1038/s41467-021-27471-6 |
|---|
| URL: | kostenfrei: Volltext: https://doi.org/10.1038/s41467-021-27471-6 |
|---|
| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-021-27471-6 |
|---|
| | DOI: https://doi.org/10.1038/s41467-021-27471-6 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | RNA |
|---|
| | RNA decay |
|---|
| K10plus-PPN: | 1817582186 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
| |
|
|---|
| | |
|---|
| Lokale URL UB: |  Zum Volltext |
|---|
RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation / Poetz, Fabian [VerfasserIn]; 09 December 2021 (Online-Ressource)
