Marathon-induced cardiac strain as model for the evaluation of diagnostic microRNAs for acute myocardial infarction

• Verfasst von: Shirvani-Samani, Omid [VerfasserIn]
• Verfasst von: Scherr, Johannes [VerfasserIn]
• Verfasst von: Kayvanpour, Elham [VerfasserIn]
• Verfasst von: Haas, Jan [VerfasserIn]
• Verfasst von: Lehmann, David Hermann [VerfasserIn]
• Verfasst von: Gi, Weng-Tein [VerfasserIn]
• Verfasst von: Frese, Karen S. [VerfasserIn]
• Verfasst von: Nietsch, Rouven [VerfasserIn]
• Verfasst von: Fehlmann, Tobias [VerfasserIn]
• Verfasst von: Sandke, Steffi [VerfasserIn]
• Verfasst von: Weis, Tanja [VerfasserIn]
• Verfasst von: Keller, Andreas [VerfasserIn]
• Verfasst von: Katus, Hugo [VerfasserIn]
• Verfasst von: Halle, Martin [VerfasserIn]
• Verfasst von: Frey, Norbert [VerfasserIn]
• Verfasst von: Meder, Benjamin [VerfasserIn]
• Verfasst von: Sedaghat-Hamedani, Farbod [VerfasserIn]
• Titel: Marathon-induced cardiac strain as model for the evaluation of diagnostic microRNAs for acute myocardial infarction
• Verf.angabe: Omid Shirvani Samani, Johannes Scherr, Elham Kayvanpour, Jan Haas, David H. Lehmann, Weng-Tein Gi, Karen S. Frese, Rouven Nietsch, Tobias Fehlmann, Steffi Sandke, Tanja Weis, Andreas Keller, Hugo A. Katus, Martin Halle, Norbert Frey, Benjamin Meder and Farbod Sedaghat-Hamedani
• Jahr: 2022
• Umfang: 20 S.
• Fussnoten: Published: 21 December 2021 ; Gesehen am 27.09.2022
• Titel Quelle: Enthalten in: Journal of Clinical Medicine
• Ort Quelle: Basel : MDPI, 2012
• Jahr Quelle: 2022
• Band/Heft Quelle: 11(2022), 1, Artikel-ID 5, Seite 1-20
• ISSN Quelle: 2077-0383
• DOI: doi:10.3390/jcm11010005
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: biomarker
• Sach-SW: exercise
• Sach-SW: marathon running
• Sach-SW: microRNAs
• Sach-SW: myocardial infarction
• Sach-SW: troponin
• K10plus-PPN: 1817602403

Abstract

Background: The current gold standard biomarker for myocardial infarction (MI), cardiac troponin (cTn), is recognized for its high sensitivity and organ specificity; however, it lacks diagnostic specificity. Numerous studies have introduced circulating microRNAs as potential biomarkers for MI. This study investigates the MI-specificity of these serum microRNAs by investigating myocardial stress/injury due to strenuous exercise. Methods: MicroRNA biomarkers were retrieved by comprehensive review of 109 publications on diagnostic serum microRNAs for MI. MicroRNA levels were first measured by next-generation sequencing in pooled sera from runners (n = 46) before and after conducting a full competitive marathon. Hereafter, reverse transcription quantitative real-time PCR (qPCR) of 10 selected serum microRNAs in 210 marathon runners was performed (>10,000 qPCR measurements). Results: 27 potential diagnostic microRNA for MI were retrieved by the literature review. Eight microRNAs (miR-1-3p, miR-21-5p, miR-26a-5p, miR-122-5p, miR-133a-3p, miR-142-5p, miR-191-5p, miR-486-3p) showed positive correlations with cTnT in marathon runners, whereas two miRNAs (miR-134-5p and miR-499a-5p) showed no correlations. Upregulation of miR-133a-3p (p = 0.03) and miR-142-5p (p = 0.01) went along with elevated cTnT after marathon. Conclusion: Some MI-associated microRNAs (e.g., miR-133a-3p and miR-142-5p) have similar kinetics under strenuous exercise and MI as compared to cTnT, which suggests that their diagnostic specificity could be limited. In contrast, several MI-associated microRNAs (miR-26a-5p, miR-134-5p, miR-191-5p) showed different release behavior; hence, combining cTnT with these microRNAs within a multi-marker strategy may add diagnostic accuracy in MI.