Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Paul, David [VerfasserIn]   i
 Romero-Brey, Inés [VerfasserIn]   i
 Gouttenoire, Jérôme [VerfasserIn]   i
 Stoitsova, Savina [VerfasserIn]   i
 Krijnse-Locker, Jacomine [VerfasserIn]   i
 Moradpour, Darius [VerfasserIn]   i
 Bartenschlager, Ralf [VerfasserIn]   i
Titel:NS4B self-interaction through conserved C-terminal elements is required for the establishment of functional hepatitis C virus replication complexes
Verf.angabe:David Paul, Inés Romero-Brey, Jérôme Gouttenoire, Savina Stoitsova, Jacomine Krijnse-Locker, Darius Moradpour, and Ralf Bartenschlager
E-Jahr:2011
Jahr:15 July 2011
Umfang:14 S.
Fussnoten:Gesehen am 28.09.2022
Titel Quelle:Enthalten in: Journal of virology
Ort Quelle:Baltimore, Md. : Soc., 1967
Jahr Quelle:2011
Band/Heft Quelle:85(2011), 14, Seite 6963-6976
ISSN Quelle:1098-5514
Abstract:Hepatitis C virus (HCV) is an important human pathogen, persistently infecting more than 170 million individuals worldwide. Studies of the HCV life cycle have become possible with the development of cell culture systems supporting the replication of viral RNA and the production of infectious virus. However, the exact functions of individual proteins, especially of nonstructural protein 4B (NS4B), remain poorly understood. NS4B triggers the formation of specific, vesicular membrane rearrangements, referred to as membranous webs, which have been reported to represent sites of HCV RNA replication. However, the mechanism of vesicle induction is not known. In this study, a panel of 15 mutants carrying substitutions in the highly conserved NS4B C-terminal domain was generated. Five mutations had only a minor effect on replication, but two of them enhanced assembly and release of infectious virus. Ten mutants were replication defective and used for selection of pseudoreversions. Most of the pseudoreversions also localized to the highly conserved NS4B C-terminal domain and were found to restore replication competence upon insertion into the corresponding primary mutant. Importantly, pseudoreversions restoring replication competence also restored heterotypic NS4B self-interaction, which was disrupted by the primary mutation. Finally, electron microscopy analyses of membrane alterations induced by NS4B mutants revealed striking morphological abnormalities, which were restored to wild-type morphology by the corresponding pseudoreversion. These findings demonstrate the important role of the C-terminal domain in NS4B self-interaction and the formation of functional HCV replication complexes.
DOI:doi:10.1128/JVI.00502-11
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1128/JVI.00502-11
 DOI: https://doi.org/10.1128/JVI.00502-11
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Base Sequence
 Blotting, Western
 Cell Line
 Conserved Sequence
 DNA Primers
 Fluorescence Resonance Energy Transfer
 Hepacivirus
 Humans
 Mutation
 RNA, Viral
 Viral Nonstructural Proteins
 Virus Replication
K10plus-PPN:1817714317
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68969055   QR-Code
zum Seitenanfang