| |  Online-Ressource |
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| Verfasst von: | Herbst, Friederike [VerfasserIn]  |
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| | Ball, Claudia R. [VerfasserIn] |
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| | Zavidij, Oksana [VerfasserIn] |
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| | Fessler, Sylvia [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Veelken, Hendrik [VerfasserIn] |
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| | Kalle, Christof von [VerfasserIn] |
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| | Glimm, Hanno [VerfasserIn] |
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| Titel: | 10-year stability of clinical-grade serum-free γ-retroviral vector-containing medium |
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| Verf.angabe: | F. Herbst, C.R. Ball, O. Zavidij, S. Fessler, M. Schmidt, H. Veelken, C. von Kalle and H. Glimm |
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| E-Jahr: | 2011 |
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| Jahr: | [2011] |
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| Umfang: | 3 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Published: 11 November 2010 ; Gesehen am 06.10.2022 |
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| Titel Quelle: | Enthalten in: Gene therapy |
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| Ort Quelle: | London : Nature Publ. Group, 1997 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 18(2011), 2, Seite 210-212 |
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| ISSN Quelle: | 1476-5462 |
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| Abstract: | More than 10 years ago, we developed an efficient protocol for serum-free retroviral transduction of human hematopoietic stem cells derived from mobilized peripheral blood. After upscaling of the methodology, serum-free retroviral gibbon-ape leukemia virus (GALV) pseudotype PG13/LN vector supernatant produced under strict good manufacturing practice (GMP) conditions was used in the first clinical gene-marking trial in Germany. In this study, we analyzed the titer and transduction efficiency of this serum-free clinical-grade retroviral supernatant 10 years after production to evaluate the long-term stability. Long-term storage and transport on dry ice resulted in modestly decreased titers and levels of transduction efficiency in CD34+ cells ranging from 38.4 to 49.1%. We conclude that the stability of retroviral vectors in serum-free medium allows extended storage and distribution of approved clinical-grade retroviral vector stocks to distant sites in multicenter clinical trials. |
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| DOI: | doi:10.1038/gt.2010.126 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/gt.2010.126 |
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| | Volltext: https://www.nature.com/articles/gt2010126 |
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| | DOI: https://doi.org/10.1038/gt.2010.126 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Biological techniques |
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| | Viral vectors |
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| K10plus-PPN: | 1818157926 |
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| Verknüpfungen: | → Zeitschrift |
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10-year stability of clinical-grade serum-free γ-retroviral vector-containing medium / Herbst, Friederike [VerfasserIn]; [2011] (Online-Ressource)
