Immunodominance of HLA-A2-restricted Hepatitis C virus-specific CD8+ T cell responses is linked to naïve-precursor frequency

• Verfasst von: Schmidt, Julia [VerfasserIn]
• Verfasst von: Neumann-Haefelin, Christoph [VerfasserIn]
• Verfasst von: Altay, Tayibe [VerfasserIn]
• Verfasst von: Gostick, Emma [VerfasserIn]
• Verfasst von: Price, David A. [VerfasserIn]
• Verfasst von: Lohmann, Volker [VerfasserIn]
• Verfasst von: Blum, Hubert E. [VerfasserIn]
• Verfasst von: Thimme, Robert [VerfasserIn]
• Titel: Immunodominance of HLA-A2-restricted Hepatitis C virus-specific CD8+ T cell responses is linked to naïve-precursor frequency
• Verf.angabe: Julia Schmidt, Christoph Neumann-Haefelin, Tayibe Altay, Emma Gostick, David A. Price, Volker Lohmann, Hubert E. Blum, and Robert Thimme
• E-Jahr: 2011
• Jahr: 21 April 2011
• Umfang: 5 S.
• Fussnoten: Gesehen am 12.10.2022 ; Im Titel ist das Pluszeichen hochgestellt
• Titel Quelle: Enthalten in: Journal of virology
• Ort Quelle: Baltimore, Md. : Soc., 1967
• Jahr Quelle: 2011
• Band/Heft Quelle: 85(2011), 10, Seite 5232-5236
• ISSN Quelle: 1098-5514
• DOI: doi:10.1128/JVI.00093-11
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1818795094

Abstract

The impact of naïve-precursor frequency on human virus-specific CD8+ T cell immunodominance is not well understood. Using a recently developed major histocompatibility complex (MHC) class I tetramer enrichment protocol, we found a conserved hierarchy and a >10-fold difference in naïve-precursor frequencies across three HLA-A2-restricted hepatitis C virus (HCV)-specific epitopes. Importantly, the NS31406 epitope with the highest naïve-precursor frequency in healthy donors was also the most frequently targeted epitope in a large cohort of chronically HCV-infected patients, both ex vivo and after in vitro stimulation. These results indicate for the first time that immunodominance in a human viral infection is linked to naïve-precursor frequency.