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Verfasst von:Schmuck, Rosa [VerfasserIn]   i
 Warneke, Viktoria [VerfasserIn]   i
 Behrens, Hans-Michael [VerfasserIn]   i
 Simon, Eva [VerfasserIn]   i
 Weichert, Wilko [VerfasserIn]   i
 Röcken, Christoph [VerfasserIn]   i
Titel:Genotypic and phenotypic characterization of side population of gastric cancer cell lines
Verf.angabe:Rosa Schmuck, Viktoria Warneke, Hans-Michael Behrens, Eva Simon, Wilko Weichert, and Christoph Röcken
E-Jahr:2011
Jahr:[April 2011]
Umfang:13 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 12.10.2022
Titel Quelle:Enthalten in: The American journal of pathology
Ort Quelle:New York [u.a.] : Elsevier, 1925
Jahr Quelle:2011
Band/Heft Quelle:178(2011), 4 vom: Apr., Seite 1792-1804
ISSN Quelle:1525-2191
Abstract:The side population (SP) of tumor cell lines shares characteristics with tumor stem cells. The objective of this study was to phenotypically and genotypically characterize the SP of gastric cancer cell lines. SP cells were obtained from AGS and MKN45 gastric cancer cells using Hoechst 33342 staining and fluorescence-activated cell sorting. The cells were subsequently studied morphologically at cytology and immunocytochemistry, on the transcriptional level via gene array, and in cell culture using recultivation assays. Genes differentially expressed in SP cells were evaluated at immunohistochemistry in tissue samples from 486 patients with gastric cancer. The SP cells were smaller and rounder then non-SP cells. SP cells self-renewed in recultivation experiments and differentiated into SP and non-SP cells. Recultivated SP and non-SP cells exhibited distinct phenotypes in culture insofar as cell shape and colony formation. SP cells demonstrated increased levels of the stem cell markers CD133 and Musashi-1. Transcriptional analyses demonstrated that SP cells express genes that encode for stem cell properties including FZD7, HEY1, SMO, and ADAM17. It was observed that ADAM17 and FZD7 are differentially expressed in human gastric cancer, and FZD7-positive cancers are associated with significantly shorter patient survival. In conclusion, human gastric cancer cell lines enclose a phenotypically and genotypically distinct cell population with tumor stem cell features. Phenotypic characteristics of this distinct cell population are also present in gastric cancer tissue, and correlate with patient survival.
DOI:doi:10.1016/j.ajpath.2010.12.043
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.ajpath.2010.12.043
 Volltext: https://www.sciencedirect.com/science/article/pii/S0002944011000435
 DOI: https://doi.org/10.1016/j.ajpath.2010.12.043
Datenträger:Online-Ressource
Sprache:eng
Bibliogr. Hinweis:Erscheint auch als : Druck-Ausgabe: Genotypic and phenotypic characterization of side population of gastric cancer cell lines. - 2011
K10plus-PPN:1818813572
Verknüpfungen:→ Zeitschrift

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