Rational design of porous poly(ethylene glycol) films as a matrix for ssDNA immobilization and hybridization

• Verfasst von: Zhao, Zhiyong [VerfasserIn]
• Verfasst von: Das, Saunak [VerfasserIn]
• Verfasst von: Zharnikov, Michael [VerfasserIn]
• Titel: Rational design of porous poly(ethylene glycol) films as a matrix for ssDNA immobilization and hybridization
• Verf.angabe: Zhiyong Zhao, Saunak Das, Michael Zharnikov
• E-Jahr: 2022
• Jahr: 24 August 2022
• Umfang: 16 S.
• Fussnoten: Gesehen am 18.10.2022
• Titel Quelle: Enthalten in: Bioengineering
• Ort Quelle: Basel : MDPI, 2014
• Jahr Quelle: 2022
• Band/Heft Quelle: 9(2022), 9, Artikel-ID 414, Seite 1-16
• ISSN Quelle: 2306-5354
• DOI: doi:10.3390/bioengineering9090414
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: electrochemical impedance spectroscopy
• Sach-SW: poly(ethylene glycol)
• Sach-SW: ssDNA hybridization
• Sach-SW: ssDNA immobilization
• Sach-SW: STAR-PEGs
• Sach-SW: X-ray photoelectron spectroscopy
• K10plus-PPN: 1819112608

Abstract

Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on the reaction between the amine groups in the films and N-hydroxy succinimide (NHS) ester groups of the NHS-ester-decorated ssDNA. Whereas the amount of reactive amine groups in the films with the standard 1:1 composition of the precursors turned out to be too low for efficient immobilization, it could be increased noticeably using an excess (2:1) concentration of the amine-terminated precursor. The respective films retained the bioinertness of the 1:1 prototype and could be successfully decorated with probe ssDNA, resulting in porous, 3D PEG-ssDNA sensing assemblies. These assemblies exhibited high selectivity with respect to the target ssDNA strands, with a hybridization efficiency of 78-89% for the matching sequences and full inertness for non-complementary strands. The respective strategy can be applied to the fabrication of DNA microarrays and DNA sensors. As a suitable transduction technique, requiring no ssDNA labeling and showing high sensitivity in the PEG-ssDNA case, electrochemical impedance spectroscopy is suggested.