Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Zhao, Zhiyong [VerfasserIn]  |
| Das, Saunak [VerfasserIn]  |
| Zharnikov, Michael [VerfasserIn]  |
Titel: | Rational design of porous poly(ethylene glycol) films as a matrix for ssDNA immobilization and hybridization |
Verf.angabe: | Zhiyong Zhao, Saunak Das, Michael Zharnikov |
E-Jahr: | 2022 |
Jahr: | 24 August 2022 |
Umfang: | 16 S. |
Fussnoten: | Gesehen am 18.10.2022 |
Titel Quelle: | Enthalten in: Bioengineering |
Ort Quelle: | Basel : MDPI, 2014 |
Jahr Quelle: | 2022 |
Band/Heft Quelle: | 9(2022), 9, Artikel-ID 414, Seite 1-16 |
ISSN Quelle: | 2306-5354 |
Abstract: | Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on the reaction between the amine groups in the films and N-hydroxy succinimide (NHS) ester groups of the NHS-ester-decorated ssDNA. Whereas the amount of reactive amine groups in the films with the standard 1:1 composition of the precursors turned out to be too low for efficient immobilization, it could be increased noticeably using an excess (2:1) concentration of the amine-terminated precursor. The respective films retained the bioinertness of the 1:1 prototype and could be successfully decorated with probe ssDNA, resulting in porous, 3D PEG-ssDNA sensing assemblies. These assemblies exhibited high selectivity with respect to the target ssDNA strands, with a hybridization efficiency of 78-89% for the matching sequences and full inertness for non-complementary strands. The respective strategy can be applied to the fabrication of DNA microarrays and DNA sensors. As a suitable transduction technique, requiring no ssDNA labeling and showing high sensitivity in the PEG-ssDNA case, electrochemical impedance spectroscopy is suggested. |
DOI: | doi:10.3390/bioengineering9090414 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/bioengineering9090414 |
| Volltext: https://www.mdpi.com/2306-5354/9/9/414 |
| DOI: https://doi.org/10.3390/bioengineering9090414 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | electrochemical impedance spectroscopy |
| poly(ethylene glycol) |
| ssDNA hybridization |
| ssDNA immobilization |
| STAR-PEGs |
| X-ray photoelectron spectroscopy |
K10plus-PPN: | 1819112608 |
Verknüpfungen: | → Zeitschrift |
Rational design of porous poly(ethylene glycol) films as a matrix for ssDNA immobilization and hybridization / Zhao, Zhiyong [VerfasserIn]; 24 August 2022 (Online-Ressource)
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