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Verfasst von:Zhao, Zhiyong [VerfasserIn]   i
 Das, Saunak [VerfasserIn]   i
 Zharnikov, Michael [VerfasserIn]   i
Titel:Rational design of porous poly(ethylene glycol) films as a matrix for ssDNA immobilization and hybridization
Verf.angabe:Zhiyong Zhao, Saunak Das, Michael Zharnikov
E-Jahr:2022
Jahr:24 August 2022
Umfang:16 S.
Fussnoten:Gesehen am 18.10.2022
Titel Quelle:Enthalten in: Bioengineering
Ort Quelle:Basel : MDPI, 2014
Jahr Quelle:2022
Band/Heft Quelle:9(2022), 9, Artikel-ID 414, Seite 1-16
ISSN Quelle:2306-5354
Abstract:Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on the reaction between the amine groups in the films and N-hydroxy succinimide (NHS) ester groups of the NHS-ester-decorated ssDNA. Whereas the amount of reactive amine groups in the films with the standard 1:1 composition of the precursors turned out to be too low for efficient immobilization, it could be increased noticeably using an excess (2:1) concentration of the amine-terminated precursor. The respective films retained the bioinertness of the 1:1 prototype and could be successfully decorated with probe ssDNA, resulting in porous, 3D PEG-ssDNA sensing assemblies. These assemblies exhibited high selectivity with respect to the target ssDNA strands, with a hybridization efficiency of 78-89% for the matching sequences and full inertness for non-complementary strands. The respective strategy can be applied to the fabrication of DNA microarrays and DNA sensors. As a suitable transduction technique, requiring no ssDNA labeling and showing high sensitivity in the PEG-ssDNA case, electrochemical impedance spectroscopy is suggested.
DOI:doi:10.3390/bioengineering9090414
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/bioengineering9090414
 Volltext: https://www.mdpi.com/2306-5354/9/9/414
 DOI: https://doi.org/10.3390/bioengineering9090414
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:electrochemical impedance spectroscopy
 poly(ethylene glycol)
 ssDNA hybridization
 ssDNA immobilization
 STAR-PEGs
 X-ray photoelectron spectroscopy
K10plus-PPN:1819112608
Verknüpfungen:→ Zeitschrift

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