Measuring forces between two single actin filaments during bundle formation

• Verfasst von: Streichfuss, Martin [VerfasserIn]
• Verfasst von: Erbs, Friedrich Philipp Joachim Erich [VerfasserIn]
• Verfasst von: Uhrig, Kai [VerfasserIn]
• Verfasst von: Kurre, Rainer [VerfasserIn]
• Verfasst von: Clemen, Anabel E.-M. [VerfasserIn]
• Verfasst von: Böhm, Christian H. J. [VerfasserIn]
• Verfasst von: Haraszti, Tamás [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Measuring forces between two single actin filaments during bundle formation
• Verf.angabe: Martin Streichfuss, Friedrich Erbs, Kai Uhrig, Rainer Kurre, Anabel E.-M. Clemen, Christian H.J. Böhm, Tamás Haraszti, and Joachim P. Spatz
• E-Jahr: 2011
• Jahr: 12 August 2011
• Umfang: 5 S.
• Fussnoten: Gesehen am 24.10.2022
• Titel Quelle: Enthalten in: Nano letters
• Ort Quelle: Washington, DC : ACS Publ., 2001
• Jahr Quelle: 2011
• Band/Heft Quelle: 11(2011), 9, Seite 3676-3680
• ISSN Quelle: 1530-6992
• DOI: doi:10.1021/nl201630y
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1819785122

Abstract

Bundles of filamentous actin are dominant cytoskeletal structures, which play a crucial role in various cellular processes. As yet quantifying the fundamental interaction between two individual actin filaments forming the smallest possible bundle has not been realized. Applying holographic optical tweezers integrated with a microfluidic platform, we were able to measure the forces between two actin filaments during bundle formation. Quantitative analysis yields forces up to 0.2 pN depending on the concentration of bundling agents.