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Verfasst von:Schubert, Mario [VerfasserIn]   i
 Herbert, Nicolás Nathaniel [VerfasserIn]   i
 Hoffmann, Isabel [VerfasserIn]   i
 Ran, Dan [VerfasserIn]   i
 Singh, Rahul [VerfasserIn]   i
 Eckstein, Volker [VerfasserIn]   i
 Vitacolonna, Mario [VerfasserIn]   i
 Ho, Anthony Dick [VerfasserIn]   i
 Zöller, Margot [VerfasserIn]   i
Titel:Differential survival of AML subpopulations in NOD/SCID mice
Verf.angabe:Mario Schubert, Nicolás Herbert, Isabel Taubert, Dan Ran, Rahul Singh, Volker Eckstein, Mario Vitacolonna, Anthony D. Ho, Margot Zöller
E-Jahr:2011
Jahr:[February 2011]
Umfang:18 S.
Illustrationen:Illustrationen, Diagramme
Fussnoten:Gesehen am 27.10.2022
Titel Quelle:Enthalten in: Experimental hematology
Ort Quelle:Kidlington [u.a] : Elsevier, 1964
Jahr Quelle:2011
Band/Heft Quelle:volume 39, issue 2 (February 2011), Seite 250-263, e1-e4
ISSN Quelle:0531-5573
 0301-472X
Abstract:Objective - Leukemia-initiating cells can retrospectively be defined by tumorigenicity in immunodeficient mice and be characterized by surface markers. The latter still being discussed for acute myeloid leukemia (AML), nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice were used to evaluate long-time reconstitution and expansion of AML subpopulations. - Materials and Methods - Bone marrow cells from patients with AML were separated according to CD34 expression, aldehyde dehydrogenase (ALDH) activity, and divisional kinetics in comparison to cord blood−derived CD34+ hematopoietic stem cells, evaluating survival and expansion in NOD/SCID mice. The AML long-term surviving capacity of subpopulations recovered from NOD/SCID mice was confirmed by ex vivo survival. - Results - AML mononuclear cells were detected in bone marrow and spleen of NOD/SCID mice 12 weeks after transplantation. The majority of recovered cells were CD34+ and significantly more CD34+ cells were recovered after application of ALDHbright (high ALDH activity), CD34+, or slowly dividing (PKHbright) than after ALDHdim, CD34−, or fast dividing (PKHdim) cell application. CD123+, CD63+, and CD44v7+ cells were also more abundant after the transfer of ALDHbright or CD34+ AML mononuclear cells. In the spleen, large AML cell clusters were only recovered after ALDHbright, CD34+, or PKHbright cell transfer. Importantly, in secondary long-term in vitro cultures, quite exclusively CD34+ AML mononuclear cells survived and expanded. - Conclusions - Separation of ALDHbright, CD34+, or PKHbright cells enriches for AML long-term surviving capacity, which reside in the CD34+ subpopulation, as rather exclusively CD34+ cells survived and expanded in vivo and ex vivo. Long-term survival capacity may be supported by CD44v7 expression.
DOI:doi:10.1016/j.exphem.2010.10.010
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.exphem.2010.10.010
 Volltext: https://www.sciencedirect.com/science/article/pii/S0301472X10005485
 DOI: https://doi.org/10.1016/j.exphem.2010.10.010
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1820166260
Verknüpfungen:→ Zeitschrift

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