Status: Bibliographieeintrag
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| Verfasst von: | Weiss, Stefan [VerfasserIn]  |
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| | Henle, Philipp [VerfasserIn] |
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| | Roth, Wolfgang [VerfasserIn] |
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| | Bock, Rosalie [VerfasserIn] |
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| | Boeuf, Stephane [VerfasserIn] |
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| | Richter, Wiltrud [VerfasserIn] |
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| Titel: | Design and characterization of a new bioreactor for continuous ultra-slow uniaxial distraction of a three-dimensional scaffold-free stem cell culture |
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| Verf.angabe: | S. Weiss, P. Henle, W. Roth, R. Bock, S. Boeuf and W. Richter |
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| Jahr: | 2011 |
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| Umfang: | 9 S. |
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| Fussnoten: | Online veröffentlicht November 9, 2010 ; Gesehen am 18.11.2022 |
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| Titel Quelle: | Enthalten in: Biotechnology progress |
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| Ort Quelle: | Malden, MA : Wiley, 1985 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 27(2011), 1, Seite 86-94 |
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| ISSN Quelle: | 1520-6033 |
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| Abstract: | A computer controlled dynamic bioreactor for continuous ultra-slow uniaxial distraction of a scaffold-free three-dimensional (3D) mesenchymal stem cell pellet culture was designed to investigate the influence of stepless tensile strain on behavior of distinct primary cells like osteoblasts, chondroblasts, or stem cells without the influence of an artificial culture matrix. The main advantages of this device include the following capabilities: (1) Application of uniaxial ultra-slow stepless distraction within a range of 0.5-250 μm/h and real-time control of the distraction distance with high accuracy (mean error −3.4%); (2) tension strain can be applied on a 3D cell culture within a standard CO2-incubator without use of an artificial culture matrix; (3) possibility of histological investigation without loss of distraction; (4) feasibility of molecular analysis on RNA and protein level. This is the first report on a distraction device capable of applying continuous tensile strain to a scaffold-free 3D cell culture within physiological ranges of motion comparable to distraction ostegenesis in vivo. We expect the newly designed microdistraction device to increase our understanding on the regulatory mechanisms of mechanical strains on the metabolism of stem cells. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011 |
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| DOI: | doi:10.1002/btpr.510 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/btpr.510 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.510 |
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| | DOI: https://doi.org/10.1002/btpr.510 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | continuous distraction |
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| | continuous tensile strain |
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| | dynamic bioreactor |
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| | mesenchymal stem cells |
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| | tension strain |
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| | three-dimensional cell culture |
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| K10plus-PPN: | 1822884012 |
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| Verknüpfungen: | → Zeitschrift |
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Design and characterization of a new bioreactor for continuous ultra-slow uniaxial distraction of a three-dimensional scaffold-free stem cell culture / Weiss, Stefan [VerfasserIn]; 2011 (Online-Ressource)
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