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| Verfasst von: | Antolovič, Dalibor [VerfasserIn]  |
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| | Galindo Escobedo, Luis Vidal [VerfasserIn] |
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| | Carstens, Anina [VerfasserIn] |
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| | Rahbari, Nuh Nabi [VerfasserIn] |
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| | Büchler, Markus W. [VerfasserIn] |
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| | Weitz, Jürgen [VerfasserIn] |
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| | Koch, Moritz [VerfasserIn] |
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| Titel: | Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies |
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| Verf.angabe: | Dalibor Antolovic, Luis Galindo, Anina Carstens, Nuh Rahbari, Markus W. Büchler, Jürgen Weitz and Moritz Koch |
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| E-Jahr: | 2010 |
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| Jahr: | 28 April 2010 |
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| Umfang: | 8 S. |
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| Fussnoten: | Gesehen am 23.11.2022 |
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| Titel Quelle: | Enthalten in: BMC biotechnology |
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| Ort Quelle: | London : BioMed Central, 2001 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 10(2010), Artikel-ID 35, Seite 1-8 |
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| ISSN Quelle: | 1472-6750 |
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| Abstract: | Circulating tumor cells (CTC) and disseminated tumor cells (DTC) are thought to be responsible for metastasis, so the detection of CTC may serve as individual prognostic factor in patients suffering from colorectal cancer. Therefore, a series of immunomagnetic enrichment methods for CTC have been developed using a variety of monoclonal antibodies against the Epithelial Cell Adhesion Molecule (EpCAM). However, it remains unclear whether all commercially available EpCAM antibodies show the same sensitivity and specificity. Furthermore, it remains unclear which method of sample preparation and cell extraction is most suitable for immunomagnetic enrichment and detection of CTC. In this study, we aimed to investigate whether the detection of CTC by a cytokeratin 20 reverse transcriptase-polymerase chain reaction (CK20 RT-PCR) may be influenced by the use of various Epithelial Cell Adhesion Molecule (EpCAM) antibodies for immunomagnetic isolation of CTC. |
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| DOI: | doi:10.1186/1472-6750-10-35 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1186/1472-6750-10-35 |
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| | DOI: https://doi.org/10.1186/1472-6750-10-35 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Circulate Tumor Cell |
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| | Disseminate Tumor Cell |
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| | HT29 Cell |
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| | Immunomagnetic Bead |
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| | UICC Stage |
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| K10plus-PPN: | 1823276598 |
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| Verknüpfungen: | → Zeitschrift |
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Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies / Antolovič, Dalibor [VerfasserIn]; 28 April 2010 (Online-Ressource)
