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Verfasst von:Arian, Dumitru [VerfasserIn]   i
 Cló, Emiliano [VerfasserIn]   i
 Gothelf, Kurt V. [VerfasserIn]   i
 Mokhir, Andriy [VerfasserIn]   i
Titel:A nucleic acid dependent chemical photocatalysis in live human cells
Verf.angabe:Dumitru Arian, Emiliano Cló, Kurt V. Gothelf, and Andriy Mokhir
Jahr:2010
Umfang:8 S.
Fussnoten:Zuerst veröffentlicht: 23 December 2009 ; Gesehen am 25.11.2022
Titel Quelle:Enthalten in: Chemistry - a European journal
Ort Quelle:Weinheim : Wiley-VCH, 1995
Jahr Quelle:2010
Band/Heft Quelle:16(2010), 1, Seite 288-295
ISSN Quelle:1521-3765
Abstract:Only two nucleic acid directed chemical reactions that are compatible with live cells have been reported to date. Neither of these processes generate toxic species from nontoxic starting materials. Reactions of the latter type could be applied as gene-specific drugs, for example, in the treatment of cancer. We report here the first example of a chemical reaction that generates a cytotoxic drug from a nontoxic prodrug in the presence of a specific endogeneous ribonucleic acid in live mammalian cells. In this case, the prodrug is triplet oxygen and the drug is singlet oxygen. The key component of this reaction is an inert molecule (InP-2′-OMe-RNA/Q-2′-OMe-RNA; P: photosensitizer; Q: quencher), which becomes an active photosensitizer (InP-2′-OMe-RNA) in the presence of single-stranded nucleic acid targets. Upon irradiation with red light, the photosensitizer produces over 6000 equivalents of toxic singlet oxygen per nucleic acid target. This reaction is highly sequence specific. To detect the generation of singlet oxygen in live cells, we prepared a membrane-permeable and water-soluble fluorescent scavenger, a derivative of 2,5-diphenylisobenzofurane. The scavenger decomposes upon reaction with singlet oxygen and this is manifested in a decrease in the fluorescence intensity. This effect can be conveniently monitored by flow cytometry.
DOI:doi:10.1002/chem.200902377
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1002/chem.200902377
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.200902377
 DOI: https://doi.org/10.1002/chem.200902377
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:live cells
 nucleic acids
 photocatalysis
 photosensitizers
 singlet oxygen
K10plus-PPN:1823602991
Verknüpfungen:→ Zeitschrift

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