PTPIP51, a positive modulator of the MAPK/Erk pathway, is upregulated in glioblastoma and interacts with 14-3-3β and PTP1B in situ

• Verfasst von: Petri, Meike Katinka [VerfasserIn]
• Verfasst von: Koch, P. [VerfasserIn]
• Verfasst von: Stenzinger, Albrecht [VerfasserIn]
• Verfasst von: Kuchelmeister, K. [VerfasserIn]
• Verfasst von: Nestler, U. [VerfasserIn]
• Verfasst von: Paradowska, A. [VerfasserIn]
• Verfasst von: Steger, K. [VerfasserIn]
• Verfasst von: Brobeil, A. [VerfasserIn]
• Verfasst von: Viard, M. [VerfasserIn]
• Verfasst von: Wimmer, M. [VerfasserIn]
• Titel: PTPIP51, a positive modulator of the MAPK/Erk pathway, is upregulated in glioblastoma and interacts with 14-3-3β and PTP1B in situ
• Verf.angabe: M. K. Petri, P. Koch, A. Stenzinger, K. Kuchelmeister, U. Nestler, A. Paradowska, K. Steger, A. Brobeil, M. Viard, M. Wimmer
• Jahr: 2011
• Umfang: 13 S.
• Fussnoten: Gesehen am 30.11.2022
• Titel Quelle: Enthalten in: Histology and histopathology
• Ort Quelle: Murcia : Francisco Hernández ; Juan F. Madrid, 1993
• Jahr Quelle: 2011
• Band/Heft Quelle: 26(2011), 12, Seite 1531-1543
• ISSN Quelle: 1699-5848
• DOI: doi:10.14670/HH-26.1531
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 14-3-3 Proteins
• Sach-SW: Adult
• Sach-SW: Aged
• Sach-SW: Brain Neoplasms
• Sach-SW: Extracellular Signal-Regulated MAP Kinases
• Sach-SW: Female
• Sach-SW: Germany
• Sach-SW: Glioblastoma
• Sach-SW: Humans
• Sach-SW: Immunohistochemistry
• Sach-SW: In Situ Hybridization
• Sach-SW: Male
• Sach-SW: MAP Kinase Signaling System
• Sach-SW: Middle Aged
• Sach-SW: Mitochondrial Proteins
• Sach-SW: Neoplasm Grading
• Sach-SW: Protein Tyrosine Phosphatase, Non-Receptor Type 1
• Sach-SW: Protein Tyrosine Phosphatases
• Sach-SW: Proto-Oncogene Proteins c-raf
• Sach-SW: Real-Time Polymerase Chain Reaction
• Sach-SW: Reverse Transcriptase Polymerase Chain Reaction
• Sach-SW: RNA, Messenger
• Sach-SW: src-Family Kinases
• Sach-SW: Up-Regulation
• Sach-SW: Young Adult
• K10plus-PPN: 182404318X

Abstract

Glioblastoma multiforme (GBM) is the most common and most malignant primary brain tumour. Protein tyrosine phosphatase interacting protein 51 (PTPIP51) is an interaction partner of 14-3-3β, which correlates with the grade of malignancy in gliomas. In this study PTPIP51 and its interacting partners 14-3-3β, PTP1B, c-Src, Raf-1 as well as EGFR were investigated in human glioblastoma. Twenty glioblastoma samples were analyzed on transcriptional and translational level by immunohistochemistry, in situ hybridization and RT-PCR. To compare PTPIP51 expression in gliomas of different malignancies, quantitative RT-PCR for grade II astrocytoma and GBM samples was employed. Additionally, we analyzed the correlation between PTPIP51 and 14-3-3β transcription, and checked for in situ interaction between PTPIP51 and 14-3-3β and PTP1B, respectively. PTPIP51 and 14-3-3β mRNA showed a tumour grade dependent upregulation in gliomas. Glioblastoma cells displayed a strong immunoreaction of PTPIP51, which co-localized with 14-3-3β and PTP1B. The duolink proximity ligation assay corroborated a direct in situ interaction of PTPIP51 with both proteins, known to interact with PTPIP51 in vitro. The in vitro interacting partners Raf-1 and c-Src showed a partial co-localization. Besides, immune cells located in capillaries or infiltrating the tumour tissue and endothelial cells of pseudoglomerular vessels revealed a high PTPIP51 expression. The upregulation of PTPIP51 and its connection with the EGFR/MAPK pathway by 14-3-3β via Raf-1 and by PTP1B via c-Src, argue for a functional role of PTPIP51 in the pathogenesis of human glioblastoma.