| Online-Ressource |
Verfasst von: | Baßler, Jochen [VerfasserIn]  |
| Kallas, Martina [VerfasserIn]  |
| Pertschy, Brigitte [VerfasserIn]  |
| Ulbrich, Cornelia [VerfasserIn]  |
| Thoms, Matthias [VerfasserIn]  |
| Hurt, Ed [VerfasserIn]  |
Titel: | The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly |
Verf.angabe: | Jochen Bassler, Martina Kallas, Brigitte Pertschy, Cornelia Ulbrich, Matthias Thoms, and Ed Hurt |
E-Jahr: | 2010 |
Jahr: | 2010 June 11 |
Umfang: | 16 S. |
Fussnoten: | Gesehen am 07.12.2022 |
Titel Quelle: | Enthalten in: Molecular cell |
Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 38(2010), 5, Seite 712-721 |
ISSN Quelle: | 1097-4164 |
Abstract: | The AAA(+)-ATPase Rea1 removes the ribosome biogenesis factor Rsa4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear export of the subunit. To do this, Rea1 utilizes a MIDAS domain to bind a conserved motif in Rsa4. Here, we show that the Rea1 MIDAS domain binds another pre-60S factor, Ytm1, via a related motif. In vivo Rea1 contacts Ytm1 before it contacts Rsa4, and its interaction with Ytm1 coincides with the exit of early pre-60S particles from the nucleolus to the nucleoplasm. In vitro, Rea1's ATPase activity triggers removal of the conserved nucleolar Ytm1-Erb1-Nop7 subcomplex from isolated early pre-60S particle. We suggest that the Rea1 AAA(+)-ATPase functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. |
DOI: | doi:10.1016/j.molcel.2010.05.024 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
DOI: https://doi.org/10.1016/j.molcel.2010.05.024 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Adenosine Triphosphatases |
| ATPases Associated with Diverse Cellular Activities |
| Cell Nucleolus |
| Microtubule-Associated Proteins |
| Nuclear Proteins |
| Protein Binding |
| Protein Structure, Tertiary |
| Recombinant Fusion Proteins |
| Ribosomal Proteins |
| Ribosome Subunits, Large, Eukaryotic |
| Saccharomyces cerevisiae |
| Saccharomyces cerevisiae Proteins |
| Two-Hybrid System Techniques |
K10plus-PPN: | 1826439358 |
Verknüpfungen: | → Zeitschrift |
¬The¬ AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly / Baßler, Jochen [VerfasserIn]; 2010 June 11 (Online-Ressource)