Fluorescence quenching of quantum dots by DNA nucleotides and amino acids1

• Verfasst von: Siegberg, Daniel Wolfgang [VerfasserIn]
• Verfasst von: Herten, Dirk-Peter [VerfasserIn]
• Titel: Fluorescence quenching of quantum dots by DNA nucleotides and amino acids1
• Verf.angabe: Daniel Siegberg and Dirk-Peter Herten
• E-Jahr: 2011
• Jahr: 30 May 2011
• Umfang: 5 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 09.12.2022
• Titel Quelle: Enthalten in: Australian journal of chemistry
• Ort Quelle: Melbourne : CSIRO, 1948
• Jahr Quelle: 2011
• Band/Heft Quelle: 64(2011), 5, Seite 512-516
• ISSN Quelle: 1445-0038
• DOI: doi:10.1071/CH10293
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Siegberg, Daniel Wolfgang: Fluorescence quenching of quantum dots by DNA nucleotides and amino acids. - 2011
• K10plus-PPN: 1826661611

Abstract

Quantum dots found widespread application in the biosciences as bright and highly photo-stable fluorescent probes, i.e. for single-particle tracking. In this work we used ensemble spectroscopy and single-molecule techniques to study the quenching of quantum dots by various biochemical compounds that are usually present in living cells and might thus influence the experiments. We found not only nucleotides such as cytosine, guanine, and thymine can significantly influence the fluorescence emission of CdSe and CdTe quantum dots, but also amino acids, like asparagine and tryptophan. Bulk studies on fluorescence quenching indicated a static quenching mechanism. Interestingly, we could also show by single-molecule fluorescence spectroscopy that quenching of the quantum dots can be irreversible, suggesting either a redox-reaction between quantum dot and quencher or strong binding of the quencher to the surface of the bio-conjugated quantum dots.