First experiences with the Spectrum Compact CE System

• Verfasst von: Burgardt, Nastasja [VerfasserIn]
• Verfasst von: Weissenberger, Melanie [VerfasserIn]
• Titel: First experiences with the Spectrum Compact CE System
• Verf.angabe: Nastasja Burgardt, Melanie Weissenberger
• Jahr: 2022
• Umfang: 17 S.
• Fussnoten: Online veröffentlicht am 19. Oktober 2021 ; Gesehen am 14.12.2022
• Titel Quelle: Enthalten in: International journal of legal medicine
• Ort Quelle: Berlin : Springer, 1922
• Jahr Quelle: 2022
• Band/Heft Quelle: 136(2022), 1, Seite 55-72
• ISSN Quelle: 1437-1596
• DOI: doi:10.1007/s00414-021-02673-1
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: DNA
• Sach-SW: Forensic genetics
• Sach-SW: Promega
• Sach-SW: Spectrum Compact CE System
• Sach-SW: STR
• K10plus-PPN: 1827025557
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

The newly released Spectrum Compact CE System by Promega is a capillary electrophoresis instrument developed for DNA-fragment separation and sequencing. In this study, its compatibility to 8 commercial short tandem repeat (STR) kits from 4 different manufacturers, reproducibility (sizing precision, accuracy and concordance) and robustness (sensitivity and mixture resolution) were tested and compared to the ABI PRISM® 310 Genetic Analyzer. The instrument was able to successfully analyse amplicons of all tested kits, proved to be as precise as claimed by manufacturer specifications and was shown to be more robust than the ABI PRISM® 310 Genetic Analyzer in some aspects. Analyses on the Spectrum Compact CE System were able to resolve peaks with length differences of 1-basepair in the short and long fragment range and mixtures of mixture ratios down to 1:30. We describe the advantages and limitations we have observed so far working with this instrument in our forensic genetics laboratory.