In vivo monitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography

• Verfasst von: Wielpütz, Mark Oliver [VerfasserIn]
• Verfasst von: Eichinger, Monika [VerfasserIn]
• Verfasst von: Zhou, Z. [VerfasserIn]
• Verfasst von: Leotta, Karin [VerfasserIn]
• Verfasst von: Hirtz, Stephanie [VerfasserIn]
• Verfasst von: Bartling, Sönke [VerfasserIn]
• Verfasst von: Semmler, Wolfhard [VerfasserIn]
• Verfasst von: Kauczor, Hans-Ulrich [VerfasserIn]
• Verfasst von: Puderbach, Michael [VerfasserIn]
• Verfasst von: Mall, Marcus A. [VerfasserIn]
• Titel: In vivo monitoring of cystic fibrosis-like lung disease in mice by volumetric computed tomography
• Verf.angabe: M.O. Wielpütz, M. Eichinger, Z. Zhou, K. Leotta, S. Hirtz, S.H. Bartling, W. Semmler, H.-U. Kauczor, M. Puderbach, M.A. Mall
• E-Jahr: 2011
• Jahr: April 08 2011
• Umfang: 11 S.
• Fussnoten: Gesehen am 16.12.2022
• Titel Quelle: Enthalten in: The European respiratory journal
• Ort Quelle: Lausanne : ERS, 1988
• Jahr Quelle: 2011
• Band/Heft Quelle: 38(2011), 5, Seite 1060-1070
• ISSN Quelle: 1399-3003
• DOI: doi:10.1183/09031936.00149810
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Computed tomography
• Sach-SW: cystic fibrosis
• Sach-SW: epithelial Na+ channel
• Sach-SW: lung disease
• Sach-SW: lung imaging
• Sach-SW: transgenic mouse model
• K10plus-PPN: 1827570083

Abstract

The onset and spontaneous development of cystic fibrosis (CF) lung disease remain poorly understood. In the present study, we used volumetric computed tomography (VCT) as a new method for longitudinal in vivo monitoring of early lesions and disease progression in CF-like lung disease in β-epithelial Na+ channel (ENaC)-transgenic (TG) mice. - Using a VCT scanner prototype (80 kV, 50 mA·s, scan time 19 s and spatial resolution 200 μm), βENaC-TG mice and wild-type (WT) littermates were examined longitudinally at 10 time-points from neonatal to adult ages, and VCT images were assessed by qualitative and quantitative morphological parameters. - We demonstrate that VCT detected early-onset airway mucus obstruction, diffuse infiltrates, atelectasis and air trapping as characteristic abnormalities in βENaC-TG mice. Furthermore, we show that early tracheal mucus obstruction predicted mortality in βENaC-TG mice and that the density of lung parenchyma was significantly reduced at all time-points in βENaC-TG compared with WT mice (median±sem -558±8 HU in WT versus -686±16 HU in βENaC-TG at 6 weeks of age; p<0.005). - Our study demonstrates that VCT is a sensitive, noninvasive technique for early detection and longitudinal monitoring of morphological abnormalities of CF-like lung disease in mice, and may thus provide a useful tool for pre-clinical in vivo evaluation of novel treatment strategies for CF.