Role of magnesium ions in DNA recognition by the EcoRV restriction endonuclease

• Verfasst von: Zahran, Mai [VerfasserIn]
• Verfasst von: Bereźniak, Tomasz [VerfasserIn]
• Verfasst von: Imhof, Petra [VerfasserIn]
• Verfasst von: Smith, Jeremy C. [VerfasserIn]
• Titel: Role of magnesium ions in DNA recognition by the EcoRV restriction endonuclease
• Verf.angabe: Mai Zahran, Tomasz Berezniak, Petra Imhof, Jeremy C. Smith
• E-Jahr: 2011
• Jahr: 5 August 2011
• Umfang: 5 S.
• Fussnoten: Gesehen am 19.12.2022
• Titel Quelle: Enthalten in: Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters
• Ort Quelle: Chichester : Wiley, 1968
• Jahr Quelle: 2011
• Band/Heft Quelle: 585(2011), 17, Seite 2739-2743
• ISSN Quelle: 1873-3468
• DOI: doi:10.1016/j.febslet.2011.07.036
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Divalent metal ions
• Sach-SW: Mg2+
• Sach-SW: Nuclease
• Sach-SW: Protein-DNA interaction
• Sach-SW: Restriction enzyme
• K10plus-PPN: 1827886544

Abstract

The restriction endonuclease EcoRV binds two magnesium ions. One of these ions, , binds to the phosphate group where the cleavage occurs and is required for catalysis, but the role of the other ion, is debated. Here, multiple independent molecular dynamics simulations suggest that is crucial for achieving a tightly bound protein-DNA complex and stabilizing a conformation that allows cleavage. In the absence of in all simulations the protein-DNA hydrogen bond network is significantly disrupted and the sharp kink at the central base pair step of the DNA, which is observed in the two-metal complex, is not present. Also, the active site residues rearrange in such a way that the formation of a nucleophile, required for DNA hydrolysis, is unlikely.