Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy

• Verfasst von: Pohling, Christoph [VerfasserIn]
• Verfasst von: Buckup, Tiago [VerfasserIn]
• Verfasst von: Pagenstecher, Axel [VerfasserIn]
• Verfasst von: Motzkus, Marcus [VerfasserIn]
• Titel: Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy
• Verf.angabe: Christoph Pohling, Tiago Buckup, Axel Pagenstecher, and Marcus Motzkus
• E-Jahr: 2011
• Jahr: 30 June 2011
• Umfang: 7 S.
• Fussnoten: Gesehen am 02.01.2023
• Titel Quelle: Enthalten in: Biomedical optics express
• Ort Quelle: Washington, DC : Optica, 2010
• Jahr Quelle: 2011
• Band/Heft Quelle: 2(2011), 8, Seite 2110-2116
• ISSN Quelle: 2156-7085
• DOI: doi:10.1364/BOE.2.002110
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Coherent antistokes Raman scattering spectroscopy
• Sach-SW: Medicine
• Sach-SW: Phase retrieval
• Sach-SW: Raman scattering
• Sach-SW: Raman spectroscopy
• Sach-SW: Spectroscopy
• K10plus-PPN: 1830193732

Abstract

The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imaginary part extraction followed by principal component analysis led to color contrast between grey and white matter as well as layers of granule and Purkinje cells. Additional quantitative information was obtained by using a decomposition algorithm. The results perfectly agree with HE stained references slides prepared separately making multiplex CARS an ideal approach for chemoselective imaging.