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Verfasst von:Großner, Tobias [VerfasserIn]   i
 Helbig, Lars [VerfasserIn]   i
 Schmidmaier, Gerhard [VerfasserIn]   i
 Haberkorn, Uwe [VerfasserIn]   i
 Gotterbarm, Tobias [VerfasserIn]   i
Titel:99mTc-polyphosphonate labelling
Titelzusatz:enhancement of a novel method for the quantification of osteogenic differentiation of MSCs in vitro
Verf.angabe:Tobias Grossner, Lars Helbig, Gerhard Schmidmaier, Uwe Haberkorn, Tobias Gotterbarm
E-Jahr:2022
Jahr:[October 2022]
Umfang:6 S.
Illustrationen:Diagramme
Fussnoten:99m im Titel ist hochgestellt ; Gesehen am 04.01.2023
Titel Quelle:Enthalten in: Injury
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1969
Jahr Quelle:2022
Band/Heft Quelle:Volume 53, Supplement 2 (October 2022), Seite S34-S39
ISSN Quelle:1879-0267
Abstract:Bone tissue engineering is a fast-growing field in regenerative medicine. Consequently, there is a high demand for new, fast and reliable methods to track and quantify the osteogenic differentiation of cells. Recently, a novel method was published to non-destructively quantify the hydroxyapatite content of monolayer and 3-dimensional mesenchymal stem cell cultures using the ability of 99mTechnetium-methylene diphosphonate (MDP), a well-established tracer in clinical nuclear medicine, to bind to newly synthesized hydroxyapatite. In the present study, two other commonly used 99mTechnetium tracers, 2,3-dicarboxypropane-1,1-diphosphonate (DPD) and hydroxydiphosphonate (HDP), were evaluated to see if they could also be used for the same purpose. Furthermore, we investigated if labelling at various timepoints influenced the effectiveness of the labelling. The results were analysed using one-factor ANOVA followed by Bonferroni post-hoc testing. This revealed a highly significant difference between the three osteogenic groups at each timepoint compared to their corresponding negative controls. However, there was no statistically significant difference between the three different tracers (MDP, DPD, HDP) in the osteogenic groups. Therefore all three tracers are of similar value when quantifying the extracellular hydroxylapatite content in osteogenic stem cells cultures.
DOI:doi:10.1016/j.injury.2020.12.017
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.injury.2020.12.017
 Volltext: https://www.sciencedirect.com/science/article/pii/S0020138320310482
 DOI: https://doi.org/10.1016/j.injury.2020.12.017
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:2,3-Dicarboxypropane-1,1-diphosphonate
 Hydroxyapatite quantification
 Hydroxydiphosphonate
 Methylene diphosphonate
 Osteogenic differentiation
 Technetium
K10plus-PPN:1830365533
Verknüpfungen:→ Zeitschrift

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