Balancing the length of the distal tip by septins is key for stability and signalling function of primary cilia

• Verfasst von: Kanamaru, Taishi [VerfasserIn]
• Verfasst von: Neuner, Annett [VerfasserIn]
• Verfasst von: Kurtulmus, Bahtiyar [VerfasserIn]
• Verfasst von: Pereira, Gislene [VerfasserIn]
• Titel: Balancing the length of the distal tip by septins is key for stability and signalling function of primary cilia
• Verf.angabe: Taishi Kanamaru, Annett Neuner, Bahtiyar Kurtulmus & Gislene Pereira
• Jahr: 2022
• Umfang: 19 S.
• Fussnoten: Published online 22 November 2021 ; Gesehen am 04.01.2023
• Titel Quelle: Enthalten in: European Molecular Biology OrganizationThe EMBO journal
• Ort Quelle: Heidelberg : EMBO Press, 1982
• Jahr Quelle: 2022
• Band/Heft Quelle: 41(2022), Artikel-ID e108843, Seite 1-19
• ISSN Quelle: 1460-2075
• DOI: doi:10.15252/embj.2021108843
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cilia length/segmentation
• Sach-SW: KIF7
• Sach-SW: septins
• Sach-SW: sonic hedgehog signalling
• Sach-SW: transition zone
• K10plus-PPN: 1830366637
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Abstract Primary cilia are antenna-like organelles required for signalling transduction. How cilia structure is mechanistically maintained at steady-state to promote signalling is largely unknown. Here, we define that mammalian primary cilia axonemes are formed by proximal segment (PS) and distal segment (DS) delineated by tubulin polyglutamylation-rich and -poor regions, respectively. The analysis of proximal/distal segmentation indicated that perturbations leading to cilia over-elongation influenced PS or DS length with a different impact on cilia behaviour. We identified septins as novel repressors of DS growth. We show that septins control the localisation of MKS3 and CEP290 required for a functional transition zone (TZ), and the cilia tip accumulation of the microtubule-capping kinesin KIF7, a cilia-growth inhibitor. Live-cell imaging and analysis of sonic-hedgehog (SHH) signalling activation established that DS over-extension increased cilia ectocytosis events and decreased SHH activation. Our data underlines the importance of understanding cilia segmentation for length control and cilia-dependent signalling.