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Status: Bibliographieeintrag

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Verfasst von:Wohlgemuth, Lisa [VerfasserIn]   i
 Stratmann, Alexander Elias Paul [VerfasserIn]   i
 Münnich, Frederik [VerfasserIn]   i
 Bernhard, Stefan [VerfasserIn]   i
 Thomaß, Bertram Dietrich [VerfasserIn]   i
 Münnich, Finn [VerfasserIn]   i
 Mohamed, Adam Omar Khalaf [VerfasserIn]   i
 Mannes, Marco [VerfasserIn]   i
 Schmidt, Christoph Quirin [VerfasserIn]   i
 Nilsson Ekdahl, Kristina [VerfasserIn]   i
 Nilsson, Bo [VerfasserIn]   i
 Fauler, Michael [VerfasserIn]   i
 Föhr, Karl Josef [VerfasserIn]   i
 Huber-Lang, Markus [VerfasserIn]   i
 Messerer, David Alexander Christian [VerfasserIn]   i
Titel:Modulation of neutrophil activity by soluble complement cleavage products
Titelzusatz:an in-depth analysis
Verf.angabe:Lisa Wohlgemuth, Alexander Elias Paul Stratmann, Frederik Münnich, Stefan Bernhard, Bertram Dietrich Thomaß, Finn Münnich, Adam Omar Khalaf Mohamed, Marco Mannes, Christoph Quirin Schmidt, Kristina Nilsson Ekdahl, Bo Nilsson, Michael Fauler, Karl Josef Föhr, Markus Huber-Lang, David Alexander and Christian Messerer
E-Jahr:2022
Jahr:20 October 2022
Umfang:17 S.
Fussnoten:Gesehen am 11.01.2023
Titel Quelle:Enthalten in: Cells
Ort Quelle:Basel : MDPI, 2012
Jahr Quelle:2022
Band/Heft Quelle:11(2022), 20, Artikel-ID 3297, Seite 1-17
ISSN Quelle:2073-4409
Abstract:The cellular and fluid phase-innate immune responses of many diseases predominantly involve activated neutrophil granulocytes and complement factors. However, a comparative systematic analysis of the early impact of key soluble complement cleavage products, including anaphylatoxins, on neutrophil granulocyte function is lacking. Neutrophil activity was monitored by flow cytometry regarding cellular (electro-)physiology, cellular activity, and changes in the surface expression of activation markers. The study revealed no major effects induced by C3a or C4a on neutrophil functions. By contrast, exposure to C5a or C5a des-Arg stimulated neutrophil activity as reflected in changes in membrane potential, intracellular pH, glucose uptake, and cellular size. Similarly, C5a and C5a des-Arg but no other monitored complement cleavage product enhanced phagocytosis and reactive oxygen species generation. C5a and C5a des-Arg also altered the neutrophil surface expression of several complement receptors and neutrophil activation markers, including C5aR1, CD62L, CD10, and CD11b, among others. In addition, a detailed characterization of the C5a-induced effects was performed with a time resolution of seconds. The multiparametric response of neutrophils was further analyzed by a principal component analysis, revealing CD11b, CD10, and CD16 to be key surrogates of the C5a-induced effects. Overall, we provide a comprehensive insight into the very early interactions of neutrophil granulocytes with activated complement split products and the resulting neutrophil activity. The results provide a basis for a better and, importantly, time-resolved and multiparametric understanding of neutrophil-related (patho-)physiologies.
DOI:doi:10.3390/cells11203297
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/cells11203297
 Volltext: https://www.mdpi.com/2073-4409/11/20/3297
 DOI: https://doi.org/10.3390/cells11203297
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:anaphylatoxins
 complement system
 inflammation
 innate immunity
 neutrophils
K10plus-PPN:1830818392
Verknüpfungen:→ Zeitschrift

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