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Verfasst von:Michalaki, Anna [VerfasserIn]   i
 McGivern, Allan Robert [VerfasserIn]   i
 Poschet, Gernot [VerfasserIn]   i
 Büttner, Michael [VerfasserIn]   i
 Altenburger, Rolf [VerfasserIn]   i
 Grintzalis, Konstantinos [VerfasserIn]   i
Titel:The effects of single and combined stressors on daphnids - enzyme markers of physiology and metabolomics validate the impact of pollution
Verf.angabe:Anna Michalaki, Allan Robert McGivern, Gernot Poschet, Michael Büttner, Rolf Altenburger and Konstantinos Grintzalis
E-Jahr:2022
Jahr:12 October 2022
Umfang:14 S.
Fussnoten:Gesehen am 13.01.2023
Titel Quelle:Enthalten in: Toxics
Ort Quelle:Basel : MDPI, 2013
Jahr Quelle:2022
Band/Heft Quelle:10(2022), 10, Artikel-ID 604, Seite 1-14
ISSN Quelle:2305-6304
Abstract:The continuous global increase in population and consumption of resources due to human activities has had a significant impact on the environment. Therefore, assessment of environmental exposure to toxic chemicals as well as their impact on biological systems is of significant importance. Freshwater systems are currently under threat and monitored; however, current methods for pollution assessment can neither provide mechanistic insight nor predict adverse effects from complex pollution. Using daphnids as a bioindicator, we assessed the impact in acute exposures of eight individual chemicals and specifically two metals, four pharmaceuticals, a pesticide and a stimulant, and their composite mixture combining phenotypic, biochemical and metabolic markers of physiology. Toxicity levels were in the same order of magnitude and significantly enhanced in the composite mixture. Results from individual chemicals showed distinct biochemical responses for key enzyme activities such as phosphatases, lipase, peptidase, β-galactosidase and glutathione-S-transferase. Following this, a more realistic mixture scenario was assessed with the aforementioned enzyme markers and a metabolomic approach. A clear dose-dependent effect for the composite mixture was validated with enzyme markers of physiology, and the metabolomic analysis verified the effects observed, thus providing a sensitive metrics in metabolite perturbations. Our study highlights that sensitive enzyme markers can be used in advance on the design of metabolic and holistic assays to guide the selection of chemicals and the trajectory of the study, while providing mechanistic insight. In the future this could prove to become a useful tool for understanding and predicting freshwater pollution.
DOI:doi:10.3390/toxics10100604
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/toxics10100604
 Volltext: https://www.mdpi.com/2305-6304/10/10/604
 DOI: https://doi.org/10.3390/toxics10100604
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:<i>Daphnia magna</i>
 biochemical markers
 combined stressors
 metabolomics
 mixture toxicology
 mortality
K10plus-PPN:1831059118
Verknüpfungen:→ Zeitschrift

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