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Verfasst von:Korkmaz-İçöz, Sevil [VerfasserIn]   i
 Sistori, Gianluca [VerfasserIn]   i
 Loganathan, Sivakkanan [VerfasserIn]   i
 Sayour, Alex Ali [VerfasserIn]   i
 Brlecic, Paige [VerfasserIn]   i
 Radovits, Tamás [VerfasserIn]   i
 Brune, Maik [VerfasserIn]   i
 Karck, Matthias [VerfasserIn]   i
 Szabó, Gábor [VerfasserIn]   i
Titel:Bone marrow culture-derived conditioned medium recovers endothelial function of vascular grafts following in vitro ischemia/reperfusion injury in diabetic rats
Verf.angabe:Sevil Korkmaz-Icöz, Gianluca Sistori, Sivakkanan Loganathan, Alex Ali Sayour, Paige Brlecic, Tamás Radovits, Maik Brune, Matthias Karck, and Gábor Szabó
E-Jahr:2022
Jahr:14 October 2022
Umfang:15 S.
Fussnoten:Gesehen am 16.01.2023
Titel Quelle:Enthalten in: Stem cells international
Ort Quelle:London [u.a.] : Sage-Hindawi, 2010
Jahr Quelle:2022
Band/Heft Quelle:2022(2022), Artikel-ID e7019088, Seite 1-15
ISSN Quelle:1687-9678
Abstract:Ischemia/reperfusion injury (IRI) remains a challenge in coronary artery bypass grafting (CABG). Diabetic patients with coronary artery disease are more likely to require CABG and therefore run a high risk for cardiovascular complications. Conditioned medium (CM) from bone marrow-derived mesenchymal stem cells has been shown to have beneficial effects against IRI. We hypothesized that adding CM to physiological saline protects vascular grafts from IRI in diabetic rats. Bone-marrow derived cells were isolated from nondiabetic rat femurs/tibias, and CM was generated. As we previously reported, CM contains 23 factors involved in inflammation, oxidative stress, and apoptosis. DM was induced by streptozotocin administration. Eight weeks later, to measure vascular function, aortic rings were isolated and mounted in organ bath chambers (DM group) or stored in 4°C saline, supplemented either with a vehicle (DM-IR group) or CM (DM-IR+CM group). Although DM was associated with structural changes compared to controls, there were no functional alterations. However, compared to the DM group, in the DM-IR aortas, impaired maximum endothelium-dependent vasorelaxation in response to acetylcholine (DM % vs. DM-IR % vs. DM-IR+CM %, ) was improved, caspase-3, caspase-8, caspase-9, and caspase-12 immunoreactivity was decreased, and DNA strand breakage, detected by the TUNEL assay, was reduced by CM. We present the experimental finding that the preservation of vascular grafts with CM prevents endothelial dysfunction after IRI in diabetic rats. Targeting apoptosis by CM may contribute to its protective effect.
DOI:doi:10.1155/2022/7019088
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1155/2022/7019088
 Volltext: https://www.hindawi.com/journals/sci/2022/7019088/
 DOI: https://doi.org/10.1155/2022/7019088
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1831138530
Verknüpfungen:→ Zeitschrift

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