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| Verfasst von: | Rossmann, Julia [VerfasserIn]  |
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| | Christ, Stine [VerfasserIn] |
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| | Richter, Sylvia [VerfasserIn] |
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| | Garbade, Sven [VerfasserIn] |
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| | Hoffmann, Georg F. [VerfasserIn] |
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| | Opladen, Thomas [VerfasserIn] |
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| | Okun, Jürgen G. [VerfasserIn] |
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| Titel: | Validated UPLC-MS/MS method for the analysis of vitamin B6 pyridoxal 5́-phosphate, pyridoxal, pyridoxine, pyridoxamine, and pyridoxic acid in human cerebrospinal fluid |
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| Verf.angabe: | Julia Rossmann, Stine Christ, Sylvia Richter, Sven Friedrich Garbade, Georg Friedrich Hoffmann, Thomas Opladen, Jürgen Günther Okun |
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| E-Jahr: | 2022 |
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| Jahr: | 14 October 2022 |
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| Umfang: | 9 S. |
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| Fussnoten: | Gesehen am 18.01.2023 |
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| Titel Quelle: | Enthalten in: Journal of chromatography / B |
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| Ort Quelle: | New York, NY [u.a.] : Science Direct, 1977 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 1212(2022), Artikel-ID 123503, Seite 1-9 |
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| ISSN Quelle: | 1873-376X |
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| Abstract: | Vitamin B6 and its metabolites play a crucial role in the development and interaction of brain metabolism. Following diagnostic improvements additional inherited disorders in vitamin B6 metabolism have been identified, most of them leading to a severe epileptic disorder accompanied by progressive neurological deficits including intellectual disability and microcephaly. Since early treatment can improve the outcome, fast and reliable detection of metabolic biomarkers is important. Therefore, the analysis of vitamin B6 metabolites has become increasingly important, but is, however, still challenging and limited to a few specialized laboratories. Until today, vitamin B6 metabolites are measured by liquid chromatography tandem mass spectrometry (LC-MS/MS) using trichloroacetic acid for protein precipitation. In this work, we present the development and validation of a new, accurate and reliable method for analysis and quantification of the vitamin B6 vitamers pyridoxal 5́-phosphate (PLP), pyridoxal (PL), pyridoxine (PN), pyridoxamine (PM) and pyridoxic acid (PA) in human CSF samples using acetonitrile for protein precipitation. |
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| DOI: | doi:10.1016/j.jchromb.2022.123503 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.jchromb.2022.123503 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1570023222004081 |
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| | DOI: https://doi.org/10.1016/j.jchromb.2022.123503 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cerebrospinal fluid CSF |
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| | Pyridoxal 5́-phosphate |
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| | Pyridoxine-dependent epilepsy |
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| | UPLC-ESI-MS/MS |
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| | Vitamin B |
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| K10plus-PPN: | 1831416956 |
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| Verknüpfungen: | → Zeitschrift |
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Validated UPLC-MS/MS method for the analysis of vitamin B6 pyridoxal 5́-phosphate, pyridoxal, pyridoxine, pyridoxamine, and pyridoxic acid in human cerebrospinal fluid / Rossmann, Julia [VerfasserIn]; 14 October 2022 (Online-Ressource)
