Status: Bibliographieeintrag
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| Verfasst von: | Tittelmeier, Jessica [VerfasserIn]  |
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| | Druffel-Augustin, Silke [VerfasserIn] |
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| | Alik, Ania [VerfasserIn] |
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| | Melki, Ronald [VerfasserIn] |
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| | Nussbaum-Krammer, Carmen [VerfasserIn] |
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| Titel: | Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM |
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| Verf.angabe: | Jessica Tittelmeier, Silke Druffel-Augustin, Ania Alik, Ronald Melki & Carmen Nussbaum-Krammer |
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| E-Jahr: | 2022 |
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| Jahr: | 08 December 2022 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 23.01.2023 |
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| Titel Quelle: | Enthalten in: Communications biology |
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| Ort Quelle: | London : Springer Nature, 2018 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 5(2022), Artikel-ID 1345, Seite 1-13 |
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| ISSN Quelle: | 2399-3642 |
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| Abstract: | Synucleinopathies are a heterogenous group of neurodegenerative diseases characterized by the progressive accumulation of pathological α-synuclein (α-Syn). The importance of structural polymorphism of α-Syn assemblies for distinct synucleinopathies and their progression is increasingly recognized. However, the underlying mechanisms are poorly understood. Here we use fluorescence lifetime imaging microscopy (FLIM) to investigate seeded aggregation of α-Syn in a biosensor cell line. We show that conformationally distinct α-Syn polymorphs exhibit characteristic fluorescence lifetimes. FLIM further revealed that α-Syn polymorphs were differentially processed by cellular clearance pathways, yielding fibrillar species with increased seeding capacity. Thus, FLIM is not only a powerful tool to distinguish different amyloid structures, but also to monitor the dynamic process of amyloid remodeling by the cellular environment. Our data suggest that the accumulation of highly seeding competent degradation products for particular polymorphs may account for accelerated disease progression in some patients. |
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| DOI: | doi:10.1038/s42003-022-04289-6 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s42003-022-04289-6 |
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| | Volltext: https://www.nature.com/articles/s42003-022-04289-6 |
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| | DOI: https://doi.org/10.1038/s42003-022-04289-6 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Chaperones |
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| | Protein aggregation |
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| K10plus-PPN: | 1831778025 |
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| Verknüpfungen: | → Zeitschrift |
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Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM / Tittelmeier, Jessica [VerfasserIn]; 08 December 2022 (Online-Ressource)
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