| Online-Ressource |
Verfasst von: | Lau, Benjamin [VerfasserIn]  |
| Beine-Golovchuk, Olga [VerfasserIn]  |
| Kornprobst, Markus [VerfasserIn]  |
| Cheng, Jingdong [VerfasserIn]  |
| Kressler, Dieter [VerfasserIn]  |
| Jády, Beáta [VerfasserIn]  |
| Kiss, Tamás [VerfasserIn]  |
| Beckmann, Roland [VerfasserIn]  |
| Hurt, Ed [VerfasserIn]  |
Titel: | Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release |
Verf.angabe: | Benjamin Lau, Olga Beine-Golovchuk, Markus Kornprobst, Jingdong Cheng, Dieter Kressler, Beáta Jády, Tamás Kiss, Roland Beckmann, Ed Hurt |
E-Jahr: | 2022 |
Jahr: | 22 November 2022 |
Umfang: | 22 S. |
Fussnoten: | Gesehen am 23.01.2023 |
Titel Quelle: | Enthalten in: Cell reports |
Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
Jahr Quelle: | 2022 |
Band/Heft Quelle: | 41(2022), 8, Artikel-ID 111684, Seite 1-22 |
ISSN Quelle: | 2211-1247 |
Abstract: | Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discovered CMS1 as a null suppressor of a nop14 mutant impaired in Rrp12-Enp1 factor recruitment to the 90S. Further investigations detected Cms1 at the 18S rRNA 3′ major domain of an early 90S that carried H/ACA snR83, which is known to guide pseudouridylation at two target sites within the same subdomain. Cms1 co-precipitates with many 90S factors, but Rrp12-Enp1 encircling the 3′ major domain in the mature 90S is decreased. We suggest that Cms1 associates with the 3′ major domain during early 90S biogenesis to restrict premature Rrp12-Enp1 binding but allows snR83 to timely perform its modification role before the next 90S assembly steps coupled with Cms1 release take place. |
DOI: | doi:10.1016/j.celrep.2022.111684 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.celrep.2022.111684 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S2211124722015583 |
| DOI: https://doi.org/10.1016/j.celrep.2022.111684 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | 90S pre-ribosome |
| H/ACA |
| pre-rRNA |
| pseudouridylation |
| ribosome assembly |
| RNA modification |
| snoRNA |
| suppressor |
| yeast |
K10plus-PPN: | 1831796872 |
Verknüpfungen: | → Zeitschrift |
Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release / Lau, Benjamin [VerfasserIn]; 22 November 2022 (Online-Ressource)