| |  Online-Ressource |
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| Verfasst von: | Haag, Marvin [VerfasserIn]  |
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| | Kehrer, Jessica [VerfasserIn] |
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| | Sanchez, Cecilia P. [VerfasserIn] |
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| | Deponte, Marcel [VerfasserIn] |
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| | Lanzer, Michael [VerfasserIn] |
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| Titel: | Physiological jump in erythrocyte redox potential during Plasmodium falciparum development occurs independent of the sickle cell trait |
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| Verf.angabe: | Marvin Haag, Jessica Kehrer, Cecilia P. Sanchez, Marcel Deponte, Michael Lanzer |
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| E-Jahr: | 2022 |
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| Jahr: | 16 November 2022 |
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| Umfang: | 13 S. |
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| Fussnoten: | Online verfügbar 10 November 2022, Artikelversion 16 November 2022 ; Gesehen am 25.01.2023 |
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| Titel Quelle: | Enthalten in: Redox Biology |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 2013 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 58(2022) vom: Nov., Artikel-ID 102536, Seite 1-13 |
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| ISSN Quelle: | 2213-2317 |
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| Abstract: | The redox state of the host-parasite unit has been hypothesized to play a central role for the fitness of the intraerythrocytic blood stages of the human malaria parasite Plasmodium falciparum. In particular, hemoglobinopathies have been suggested to cause a more oxidizing environment, thereby protecting from severe malaria. Here we determined the redox potential of infected wild-type (hemoglobin AA) or sickle trait (hemoglobin AS) erythrocytes using parasite-encoded variants of the redox-sensitive green-fluorescent protein 2 (roGFP2). Our non-invasive roGFP2 single-cell measurements revealed a reducing steady-state redox potential of −304 ± 11 mV for the erythrocyte cytosol during ring-stage development and a rather sudden oxidation to −278 ± 12 mV during trophozoite-stage development around 28 h post invasion. There was no significant difference between wild-type or sickle trait erythrocytes regarding the stage dependence and the detected increase of the redox potential during the intraerythrocytic life cycle. The steady-state redox potential of the parasite cytosol, between −304 and −313 mV, was highly reducing throughout the life cycle. The redox potential in the parasitophorous vacuole at the interface between the secretory pathway and the erythrocyte was −284 ± 10 mV and remained stable during trophozoite-stage development with implications for the export of disulfide-containing proteins. In summary, P. falciparum blood stage development from the late ring to the early trophozoite stage causes a physiological jump in erythrocyte redox potential irrespective of the presence or absence of hemoglobin S. |
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| DOI: | doi:10.1016/j.redox.2022.102536 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.redox.2022.102536 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2213231722003081 |
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| | DOI: https://doi.org/10.1016/j.redox.2022.102536 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Erythrocyte redox potential |
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| | Genetically encoded redox sensors |
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| | Glutathione |
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| | Oxidation |
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| | Plasmodium falciparum infected red blood cells |
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| | Reduction |
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| | roGFP2 |
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| | Secretory pathway |
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| | Sickle cell trait |
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| K10plus-PPN: | 1832389111 |
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| Verknüpfungen: | → Zeitschrift |
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Physiological jump in erythrocyte redox potential during Plasmodium falciparum development occurs independent of the sickle cell trait / Haag, Marvin [VerfasserIn]; 16 November 2022 (Online-Ressource)
