Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Garg, Jaspal [VerfasserIn]  |
| Sporkova, Alexandra [VerfasserIn]  |
| Hecker, Markus [VerfasserIn]  |
| Korff, Thomas [VerfasserIn]  |
Titel: | Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids |
Verf.angabe: | Jaspal Garg, Alexandra Sporkova, Markus Hecker and Thomas Korff |
Jahr: | 2023 |
Umfang: | 15 S. |
Fussnoten: | Online veröffentlicht: 28 December 2022 ; Gesehen am 01.02.2023 |
Titel Quelle: | Enthalten in: Cells |
Ort Quelle: | Basel : MDPI, 2012 |
Jahr Quelle: | 2023 |
Band/Heft Quelle: | 12(2023), 1, Artikel-ID 128, Seite 1-15 |
ISSN Quelle: | 2073-4409 |
Abstract: | Analyses of G-protein-mediated contraction and relaxation of vascular smooth muscle cells (VSMCs) are usually hampered by a rigid growth surface and culture conditions promoting cell proliferation and a less contractile phenotype. Our studies indicated that mouse aortic VSMCs cultured in three-dimensional spheroids acquire a quiescent contractile status while decreasing the baseline G-protein-dependent inositolphosphate formation and increasing the expression of endothelin receptor type A (Ednra). Endothelin-1 (ET-1) promoted inositolphosphate formation in VSMC spheroids, but not in VSMCs cultured under standard conditions. To trace ET-1-mediated contraction of VSMC spheroids, we developed an assay by adhering them to collagen hydrogels and recording structural changes by time-lapse microscopy. Under these conditions, mouse and human VSMC spheroids contracted upon treatment with ET-1 and potassium chloride or relaxed in response to caffeine and the prostacyclin analogue Iloprost. ET-1 activated AKT-, MKK1-, and MKK3/6-dependent signaling cascades, which were inhibited by an overexpressing regulator of G-protein signaling 5 (Rgs5) to terminate the activity of Gα subunits. In summary, culture of VSMCs in three-dimensional spheroids lowers baseline G-protein activity and enables analyses of both contraction and relaxation of mouse and human VSMCs. This model serves as a simple and versatile tool for drug testing and investigating G-protein-depending signaling. |
DOI: | doi:10.3390/cells12010128 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cells12010128 |
| Volltext: https://www.mdpi.com/2073-4409/12/1/128 |
| DOI: https://doi.org/10.3390/cells12010128 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | contraction |
| G-protein signaling |
| MAPK |
| relaxation |
| RGS |
| VSMC phenotype |
K10plus-PPN: | 183290959X |
Verknüpfungen: | → Zeitschrift |
Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids / Garg, Jaspal [VerfasserIn]; 2023 (Online-Ressource)
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