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Status: Bibliographieeintrag

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Verfasst von:Garg, Jaspal [VerfasserIn]   i
 Sporkova, Alexandra [VerfasserIn]   i
 Hecker, Markus [VerfasserIn]   i
 Korff, Thomas [VerfasserIn]   i
Titel:Tracing G-protein-mediated contraction and relaxation in vascular smooth muscle cell spheroids
Verf.angabe:Jaspal Garg, Alexandra Sporkova, Markus Hecker and Thomas Korff
Jahr:2023
Umfang:15 S.
Fussnoten:Online veröffentlicht: 28 December 2022 ; Gesehen am 01.02.2023
Titel Quelle:Enthalten in: Cells
Ort Quelle:Basel : MDPI, 2012
Jahr Quelle:2023
Band/Heft Quelle:12(2023), 1, Artikel-ID 128, Seite 1-15
ISSN Quelle:2073-4409
Abstract:Analyses of G-protein-mediated contraction and relaxation of vascular smooth muscle cells (VSMCs) are usually hampered by a rigid growth surface and culture conditions promoting cell proliferation and a less contractile phenotype. Our studies indicated that mouse aortic VSMCs cultured in three-dimensional spheroids acquire a quiescent contractile status while decreasing the baseline G-protein-dependent inositolphosphate formation and increasing the expression of endothelin receptor type A (Ednra). Endothelin-1 (ET-1) promoted inositolphosphate formation in VSMC spheroids, but not in VSMCs cultured under standard conditions. To trace ET-1-mediated contraction of VSMC spheroids, we developed an assay by adhering them to collagen hydrogels and recording structural changes by time-lapse microscopy. Under these conditions, mouse and human VSMC spheroids contracted upon treatment with ET-1 and potassium chloride or relaxed in response to caffeine and the prostacyclin analogue Iloprost. ET-1 activated AKT-, MKK1-, and MKK3/6-dependent signaling cascades, which were inhibited by an overexpressing regulator of G-protein signaling 5 (Rgs5) to terminate the activity of Gα subunits. In summary, culture of VSMCs in three-dimensional spheroids lowers baseline G-protein activity and enables analyses of both contraction and relaxation of mouse and human VSMCs. This model serves as a simple and versatile tool for drug testing and investigating G-protein-depending signaling.
DOI:doi:10.3390/cells12010128
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/cells12010128
 Volltext: https://www.mdpi.com/2073-4409/12/1/128
 DOI: https://doi.org/10.3390/cells12010128
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:contraction
 G-protein signaling
 MAPK
 relaxation
 RGS
 VSMC phenotype
K10plus-PPN:183290959X
Verknüpfungen:→ Zeitschrift

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