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| Verfasst von: | Schott, Johanna [VerfasserIn]  |
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| | Reitter, Sonja [VerfasserIn] |
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| | Lindner, Doris [VerfasserIn] |
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| | Grosser, Jan [VerfasserIn] |
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| | Bruer, Marius [VerfasserIn] |
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| | Shenoy, Anjana [VerfasserIn] |
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| | Geiger, Tamar [VerfasserIn] |
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| | Mathes, Arthur [VerfasserIn] |
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| | Dobreva, Gergana [VerfasserIn] |
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| | Stoecklin, Georg [VerfasserIn] |
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| Titel: | Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density |
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| Verf.angabe: | Johanna Schott, Sonja Reitter, Doris Lindner, Jan Grosser, Marius Bruer, Anjana Shenoy, Tamar Geiger, Arthur Mathes, Gergana Dobreva and Georg Stoecklin |
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| E-Jahr: | 2021 |
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| Jahr: | 03 September 2021 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 01.02.2023 |
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| Titel Quelle: | Enthalten in: Nature methods |
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| Ort Quelle: | London [u.a.] : Nature Publishing Group, 2004 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 18(2021) vom: Sept., Seite 1068-1074 |
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| ISSN Quelle: | 1548-7105 |
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| Abstract: | In general, mRNAs are assumed to be loaded with ribosomes instantly upon entry into the cytoplasm. To measure ribosome density (RD) on nascent mRNA, we developed nascent Ribo-Seq by combining Ribo-Seq with progressive 4-thiouridine labeling. In mouse macrophages, we determined experimentally the lag between the appearance of nascent mRNA and its association with ribosomes, which was calculated to be 20-22 min for bulk mRNA. In mouse embryonic stem cells, nRibo-Seq revealed an even stronger lag of 35-38 min in ribosome loading. After stimulation of macrophages with lipopolysaccharide, the lag between cytoplasmic and translated mRNA leads to uncoupling between input and ribosome-protected fragments, which gives rise to distorted RD measurements under conditions where mRNA amounts are far from steady-state expression. As a result, we demonstrate that transcriptional changes affect RD in a passive way. |
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| DOI: | doi:10.1038/s41592-021-01250-z |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41592-021-01250-z |
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| | Volltext: https://www.nature.com/articles/s41592-021-01250-z |
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| | DOI: https://doi.org/10.1038/s41592-021-01250-z |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Gene expression analysis |
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| | RNA |
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| | Statistical methods |
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| | Translation |
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| K10plus-PPN: | 1832925986 |
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| Verknüpfungen: | → Zeitschrift |
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Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density / Schott, Johanna [VerfasserIn]; 03 September 2021 (Online-Ressource)
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