| |  Online-Ressource |
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| Verfasst von: | Eismann, Lena [VerfasserIn]  |
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| | Fijalkowski, Igor [VerfasserIn] |
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| | Galmozzi, Carla Verónica [VerfasserIn] |
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| | Koubek, Jiří [VerfasserIn] |
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| | Tippmann, Frank [VerfasserIn] |
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| | Van Damme, Petra [VerfasserIn] |
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| | Kramer, Günter [VerfasserIn] |
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| Titel: | Selective ribosome profiling reveals a role for SecB in the co-translational inner membrane protein biogenesis |
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| Verf.angabe: | Lena Eismann, Igor Fijalkowski, Carla Verónica Galmozzi, Jiří Koubek, Frank Tippmann, Petra Van Damme, and Günter Kramer |
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| E-Jahr: | 2022 |
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| Jahr: | 6 December 2022 |
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| Umfang: | 20 S. |
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| Fussnoten: | Gesehen am 07.02.2023 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 41(2022), 10 vom: Dez., Artikel-ID 111776, Seite 1-14, e1-e6 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | The chaperone SecB has been implicated in de novo protein folding and translocation across the membrane, but it remains unclear which nascent polypeptides SecB binds, when during translation SecB acts, how SecB function is coordinated with other chaperones and targeting factors, and how polypeptide engagement contributes to protein biogenesis. Using selective ribosome profiling, we show that SecB binds many nascent cytoplasmic and translocated proteins generally late during translation and controlled by the chaperone trigger factor. Revealing an uncharted role in co-translational translocation, inner membrane proteins (IMPs) are the most prominent nascent SecB interactors. Unlike other substrates, IMPs are bound early during translation, following the membrane targeting by the signal recognition particle. SecB remains bound until translation is terminated, and contributes to membrane insertion. Our study establishes a role of SecB in the co-translational maturation of proteins from all cellular compartments and functionally implicates cytosolic chaperones in membrane protein biogenesis. |
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| DOI: | doi:10.1016/j.celrep.2022.111776 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.celrep.2022.111776 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S221112472201659X |
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| | DOI: https://doi.org/10.1016/j.celrep.2022.111776 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | chaperone |
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| | compartment-specific ribosome profiling |
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| | membrane insertion |
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| | proteostasis |
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| | SecB |
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| | selective ribosome profiling |
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| | signal recognition particle |
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| | translocation |
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| | trigger factor |
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| K10plus-PPN: | 1833385144 |
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| Verknüpfungen: | → Zeitschrift |
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Selective ribosome profiling reveals a role for SecB in the co-translational inner membrane protein biogenesis / Eismann, Lena [VerfasserIn]; 6 December 2022 (Online-Ressource)
