Asna1/TRC40-mediated membrane insertion of tail-anchored proteins

• Verfasst von: Favaloro, Vincenzo [VerfasserIn]
• Verfasst von: Vilardi, Fabio [VerfasserIn]
• Verfasst von: Schlecht, Rainer [VerfasserIn]
• Verfasst von: Mayer, Matthias P. [VerfasserIn]
• Verfasst von: Dobberstein, Bernhard [VerfasserIn]
• Titel: Asna1/TRC40-mediated membrane insertion of tail-anchored proteins
• Verf.angabe: Vincenzo Favaloro, Fabio Vilardi, Rainer Schlecht, Matthias P. Mayer and Bernhard Dobberstein
• E-Jahr: 2010
• Jahr: 01 May 2010
• Umfang: 9 S.
• Fussnoten: Gesehen am 13.02.2023
• Titel Quelle: Enthalten in: Journal of cell science
• Ort Quelle: Cambridge : Company of Biologists Limited, 1853
• Jahr Quelle: 2010
• Band/Heft Quelle: 123(2010), 9, Seite 1522-1530
• ISSN Quelle: 1477-9137
• DOI: doi:10.1242/jcs.055970
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1834607906

Abstract

Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61β without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.